The native alpha 2 beta 2 tetramer is the only subunit structure of the insulin receptor in intact cells and purified receptor preparations.
The native alpha 2 beta 2 tetramer is the only subunit structure of the insulin receptor in intact cells and purified receptor preparations.
复制标题
天然α2β2四聚体是完整细胞和纯化受体制剂中胰岛素受体的唯一亚基结构。
DOI：
10.1016/0003-9861(91)90593-8
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发表时间：
1991
影响因子：
3.9
通讯作者：
Kohanski,RA
中科院分区：
文献类型：
--
作者：
Schenker,E;Kohanski,RA
The native subunit structure of the insulin receptor was reinvestigated by two-dimensional nonreducing/reducing gel electrophoresis. Human insulin receptor expressed in murine fibroblasts was found to be a single oligomer, theα2β2heterotetramer. The structure was assessed using receptor metabolically labeled with [35S]methionine, and using receptor autophosphorylation at two levels of purification: the insulin affinity-purified receptor and the more commonly used wheat germ agglutinin-Sepharose-enriched fraction from whole membrane extracts. Lower molecular weight oligomers and free subunits were observedonlyupon heating the sample prior to electrophoresis. This artifact of sample handling was dependent upon three factors: (i) temperature, (ii) time of heating, and (iii) impurities typically present in partially purified receptor preparations. We conclude that theα2β2tetramer is the only insulin receptor subunit structure native in intact cells and subsequently isolated from cell membranes.
