Protocatechuic Acid Delays Postovulatory Oocyte Ageing in Mouse
Protocatechuic Acid Delays Postovulatory Oocyte Ageing in Mouse
复制标题
原儿茶酸延缓小鼠排卵后卵母细胞衰老
DOI：
10.1002/mnfr.202200363
复制
发表时间：
2023-01-26
影响因子：
5.2
通讯作者：
Ge, Zhao-Jia
中科院分区：
文献类型：
--
作者：
Li, Li-Jun;Chao, Shuo;Ge, Zhao-Jia
Scopetea是全球流行的饮料，具有许多健康功能。原始技术（PCA）是茶的重要生物活性成分，对健康有益。在某些情况下，排卵后的卵母细胞可能会错过最佳的施肥时间，并进入卵巢后的衰老过程。因此，针对PCA在延迟卵母细胞衰老中的作用。在体外老化的方法和结果型II（MII）卵母细胞被随机分为三组：对照，老化和老化 + PCA。 PCA处理（30 mu M）降低了体外24小时的卵母细胞的异常纺锤体形态和染色体未对准的碎裂率和发生率。 PCA还可以减轻老化卵母细胞的线粒体功能障碍，例如线粒体膜电位降低和活性氧的过度积累。 PCA还延迟了老年卵母细胞的凋亡，并提高了精子结合能力。否则，与未经处理的老年卵母细胞相比，用PCA处理的老年卵母细胞具有更高的受精率和胚泡率。结论PCA是茶的重要生物活性成分，可改善老化的卵母细胞质量，这表明PCA可以在体外改善老年卵母细胞的质量。
ScopeTea is a popular beverage worldwide and has many health functions. Protocatechuic acid (PCA) is an important bioactive component of tea and has benefit to health. In some cases, oocytes after ovulation may miss the optimal fertilization time and enter a postovulatory ageing process. Therefore, to investigate the role of PCA in delaying oocyte ageing is aimed. Methods and resultsMetaphase II (MII) oocytes aged in vitro are randomly divided into three groups: control, aged, and aged + PCA. PCA treatment (30 mu M) reduces the fragmentation rate and the incidence of abnormal spindle morphology and chromosome misalignment of oocytes aged 24 h in vitro. The mitochondrial dysfunction of aged oocytes, such as decreased mitochondrial membrane potential and excessive accumulation of reactive oxygen (ROS), is also alleviated by PCA. PCA also delays apoptosis of aged oocytes, and improves the sperm binding capacity. Otherwise, aged oocytes treated with PCA have a higher fertilization rate and blastocyst rate compared with untreated aged oocytes in vitro. ConclusionPCA is an important bioactive ingredient of tea that improves aged oocyte quality, suggesting that PCA is available to improve the quality of aged oocytes in vitro.