S100beta protein expression: gender- and age-related daily changes.
S100beta protein expression: gender- and age-related daily changes.
复制标题
S100beta 蛋白表达：性别和年龄相关的日常变化。
DOI：
10.1007/s11064-009-9915-x
复制
发表时间：
2009
影响因子：
4.4
通讯作者：
Azmitia,EC
中科院分区：
文献类型：
--
作者：
Nogueira,MI;Abbas,SY;Campos,LGM;Allemandi,W;Lawson,P;Takada,SH;Azmitia,EC
S100β是胶质细胞主要在5-HT 1A受体激活下释放的可溶性蛋白。据报道，它是一种神经营养因子和嗜性因子，可促进发育过程中神经突的成熟和生长。这种蛋白质也在成年人大脑中轴突稳定性和长时程增强的可塑性中发挥作用。S100β快速调节神经元形态的能力引发了一个有趣的问题，即大脑中S100β水平是否存在每日节律或性别差异。为了回答这个问题，研究了成年雌性和雄性大鼠以及成年雌性CD-21和S100β −/−雌性小鼠中的S100β表达。S100β免疫反应性的闪烁计数和形态计量学分析显示，每天有节律的表达。雌性和雄性大鼠表现出相反的周期。雌性动物在休息期开始时（5：00 h）出现最高值，而雄性动物的最大值出现在运动活动期开始时（21：00 h）。这些结果证实了先前在人血清和脑脊液中的S100β评估，其报告了蛋白质作为脑损伤的生物标志物的功能（Gazzolo等人，Clin Chem 49：967-970，2003; Clin Chim Acta 330：131-133，2003; Pediatr Res 58：1170-1174，2005），还观察到与阿尔茨海默病相关的GFAP的类似行为（Fukuyama等人，Eur Neurol 46：35-38，2001）。当考虑将S100β作为健康状况的生物标志物时，应考虑这些数据。此外，研究结果提出了问题，结构或条件强加这些节奏以及观察到的性别差异的生理意义。
S100β is a soluble protein released by glial cells mainly under the activation of the 5-HT1A receptor. It has been reported as a neuro-trophic and -tropic factor that promotes neurite maturation and outgrowth during development. This protein also plays a role in axonal stability and the plasticity underlying long-term potentiation in adult brains. The ability of S100β to rapidly regulate neuronal morphology raises the interesting point of whether there are daily rhythm or gender differences in S100β level in the brain. To answer this question, the S100β expression in adult female and male rats, as well as in adult female CD-21 and S100β −/− female mice, were investigated. Scintillation counting and morphometric analysis of the immunoreactivity of S100β, showed rhythmic daily expression. The female and male rats showed opposite cycles. Females presented the highest value at the beginning of the rest phase (5:00 h), while in males the maximum value appeared in the beginning of the motor activity period (21:00 h). These results confirm previous S100β evaluations in human serum and cerebrospinal fluid reporting the protein’s function as a biomarker for brain damage (Gazzolo et al. in Clin Chem 49:967–970, 2003; Clin Chim Acta 330:131–133, 2003; Pediatr Res 58:1170–1174, 2005), similar behavior was also observed for GFAP in relation to Alzheimer Disease (Fukuyama et al. in Eur Neurol 46:35–38, 2001). The data should be taken into account when considering S100β as a biomarker of health condition. In addition, the results raise questions on which structure or condition imposes these rhythms as well as on the physiological meaning of the observed gender differences.