Growth characteristics and competitive abilities of a nucleopolyhedrovirus and an entomopoxvirus in larvae of the smaller tea tortrix, Adoxophyes honmai (Lepidoptera: Tortricidae)
Growth characteristics and competitive abilities of a nucleopolyhedrovirus and an entomopoxvirus in larvae of the smaller tea tortrix, Adoxophyes honmai (Lepidoptera: Tortricidae)
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DOI：
10.1006/bcon.2001.0988
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发表时间：
2002-01-01
影响因子：
4.2
通讯作者：
Kunimi, Y
中科院分区：
文献类型：
--
作者：
Ishii, T;Takatsuka, J;Kunimi, Y
我们研究了本米红线虫核多角体病毒 (AdhoNPV) 和本米线线虫昆虫痘病毒 (AdhoEPV) 混合感染对宿主死亡时间和病毒增殖率的影响。将 A. honmai 的新生儿和四龄幼虫同时或依次暴露于致死浓度的 AdhoNPV 和 AdhoEPV。对每种病毒产生的病毒包涵体（OB）的数量进行了量化，并在混合感染和单一感染的宿主之间进行比较。当分别将它们引入四龄幼虫时，AdhoNPV 和 AdhoEPV 分别在感染后 9 至 11 天和 18 至 24 天杀死宿主。 AdhoNPV 宿主死亡时 OB 的平均数量约为 10(10) 个多面体/昆虫，AdhoEPV 约为 10(8.5) 个球体/昆虫。同时接种 AdhoNPV 和 AdhoEPV 的四龄幼虫在注射后 9 至 11 天死亡。在这种同时混合感染处理中，AdhoEPV OB 产量显着降低，而 AdhoNPV OB 产量不受影响。然而，当新生幼虫首先暴露于 AdhoEPV，然后仅在蜕皮至第四龄后暴露于 AdhoNPV 时，AdhoNPV OB 产量减少，其最终产量下降至单次感染宿主产量的百分之几，而 AdhoEPV OB 产量不受影响。这些结果表明感染的顺序或时间显着影响两种病毒的 OB 产生。在依次感染每种病毒的幼虫中，含有两种 OB 类型的血细胞数量明显少于随机感染的预期数量。这些结果表明 AdhoNPV 和 AdhoEPV 可能由于它们之间的干扰而降低它们的适应度。 (C) 2002 年爱思唯尔科学。
We examined the effect of a mixed infection by Adoxophyes honmai nucleopolyhedrovirus (AdhoNPV) and A. honmai entomopoxvirus (AdhoEPV) on time to death of hosts and virus multiplication rate. Neonates and fourth-instar larvae of A. honmai were simultaneously or sequentially exposed to lethal concentrations of AdhoNPV and AdhoEPV. The number of viral occlusion bodies (OBs) that each virus produced was quantified and compared between mixed-infected and single-infected hosts. When each was separately introduced into fourth-instar larvae, AdhoNPV and AdhoEPV killed the host by 9 to 11 and 18 to 24 days postinfection (p.i.), respectively. The average number of OBs at time of host death was about 10(10) polyhedra/insect for AdhoNPV and 10(8.5) spheroids/insect for AdhoEPV. Fourth-instar larvae simultaneously inoculated with AdhoNPV and AdhoEPV died 9 to 11 days p.i. In this simultaneous mixed-infection treatment, AdhoEPV OB yield was significantly reduced, whereas AdhoNPV OB production was unaffected. However, when neonate larvae were exposed first to AdhoEPV and then to AdhoNPV only after molting to the fourth instar, AdhoNPV OB production was reduced and its final yield decreased to a few percent of the yield obtained in single-infected hosts, whereas AdhoEPV OB production was unaffected. These results suggest that the sequence or timing of infection significantly affects the OB production of both viruses. In larvae infected with each virus in sequence, the numbers of hemocytes containing both OB types were significantly fewer than that expected by random infection. These results suggest that AdhoNPV and AdhoEPV could reduce their fitness due to interference between them. (C) 2002 Elsevier Science.