Impact of orally-administered oligosaccharides in a murine model of food allergy
Impact of orally-administered oligosaccharides in a murine model of food allergy
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DOI：
10.1016/j.jff.2021.104643
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发表时间：
2021-07-24
影响因子：
5.6
通讯作者：
Kimura, Mariko
中科院分区：
文献类型：
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作者：
Yamashita, Hirotaka;Shigemori, Akari;Kimura, Mariko
食物过敏是一种难治性疾病，没有标准有效的治疗方法。研究发现，婴儿补充低聚糖等益生元可降低过敏性疾病的风险。棉子糖和水苏糖是豆类中鉴定的主要寡糖;这些寡糖具有调节体内平衡的特性。在这项研究中，我们探讨了使用低聚糖作为一种手段，以防止食物过敏使用卵清蛋白（OVA）致敏和挑战模型，其中降低体温和腹泻进行了评估。从第一次致敏前一周至最后一次口服OVA，在饮用水中随意给予棉子糖和水苏糖，持续5周。在我们的研究结果中，水苏糖治疗抑制过敏性腹泻，并防止OVA特异性免疫球蛋白（IG）G1升高。我们假设这些反应的抑制与调节性T细胞的作用有关，并通过肠道微生物群利用寡糖来促进。综上所述，我们的研究结果表明，每天摄入低聚糖可能对预防食物过敏有效。
Food allergy is a refractory condition for which there are no standard effective therapies. Prebiotic supplementation such as oligosaccharides in infants was found to be associated with decreased risk of allergic diseases. Raffinose and stachyose are the major oligosaccharides identified in beans; these oligosaccharides have properties of regulating homeostasis. In this study, we explored the use of oligosaccharides as a means to prevent food allergy using an ovalbumin (OVA) sensitization and challenge model in which reductions in body temperature and diarrhea were evaluated. Raffinose and stachyose were administered ad libitum in drinking water for five weeks from one week prior the first sensitization through the final oral administration of OVA. Among our findings, treatment with stachyose suppressed allergic diarrhea and prevented elevations in OVA-specific immunoglobulin (Ig)G1. We hypothesize that suppression of these responses was associated with the actions of regulatory T cells and promoted by utilization of the oligosaccharides by intestinal microbiota. Taken together, our findings suggest that daily ingestion of oligosaccharides might be effective for the prevention of food allergy.