Topography of pig retinal ganglion cells
Topography of pig retinal ganglion cells
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DOI：
10.1002/cne.20516
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发表时间：
2005-06-13
影响因子：
2.5
通讯作者：
Vecino, E
中科院分区：
文献类型：
--
作者：
García, M;Ruiz-Ederra, J;Vecino, E
本研究分析了猪视网膜神经节细胞（RGCs）的分布。通过将荧光金注射到视神经中，在体内逆行标记RGCs。分析RGC密度和RGC在索马大小方面的分布。根据细胞密度和索马大小分析RGC的分布，确定了猪视网膜的不同区域：在视网膜中央，我们发现了一个高密度的水平RGC带，位于视盘背侧。此外，在该区域，观察到具有小索马尺寸的RCG的比例高。从中央到周边视网膜，我们观察到RGC密度的降低，以及更大的胞体的RGC的存在。本研究的结果应被证明是有用的基础，为未来的研究与猪视网膜作为一个模型，在眼科研究。该研究还强调了用逆行示踪剂特异性标记RGC群体的必要性，以便精确量化与实验治疗相关的细胞群体的变化。(c)2005 Wiley-Liss，Inc.
In the present work we analyzed the distribution of retinal ganglion cells (RGCs) in the pig retina. RGCs were retrogradely labeled in vivo by injecting Fluoro-Gold into the optic nerve. RGC density and the distribution of RGCs in terms of soma size were analyzed. Different regions of the porcine retina were identified following analysis of the distribution of RGCs in terms of cell density and soma size: in the central retina, we found a high-density horizontal RGC band lying dorsal to the optic disc. Moreover, in this region, a high proportion of RCGs with small soma size was observed. From the central to the more peripheral retina, we observed a decrease in RGC density, together with a greater presence of RGCs with larger somas. The results of this study should prove to be useful as a foundation for future studies with the porcine retina as a model in ophthalmic research. The study also highlights the necessity to label the RGC population specifically with retrograde tracers in order to quantify precisely alterations in the cell population associated with experimental treatments. (c) 2005 Wiley-Liss, Inc.