Late Embryogenesis Abundant (LEA) protein gene regulation during Arabidopsis seed maturation
Late Embryogenesis Abundant (LEA) protein gene regulation during Arabidopsis seed maturation
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DOI：
10.1078/0176-1617-00353
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发表时间：
2001-04-01
影响因子：
4.3
通讯作者：
Aspart, L
中科院分区：
文献类型：
--
作者：
Delseny, M;Bies-Etheve, N;Aspart, L
本文综述了我们对拟南芥种子成熟阶段基因调控的部分研究。本质上，使用了三种互补策略。第一个包括通过对干种子 cDNA 文库中的 EST 进行随机测序，并将其频率与未成熟 cDNA 文库中的频率进行比较，来识别在此期间表达的基因。第二种策略侧重于详细分析编码 I 类 LEA 蛋白的一组特定基因（即 Em 基因）的表达，并分析其启动子。最后，我们评估了多种 LEA 基因在各种调控突变体中的表达，包括 abi3、lec1 和 abi5。总之，我们的结果说明了表达模式的复杂性以及各种因素的相互作用，从而定义了几种不同的调控途径。
This paper reviews part of our studies on gene regulation during the Arabidopsis seed maturation phase. Essentially, three complementary strategies have been used. The first one consisted in identifying genes expressed during this period by random sequencing of EST from a dry seed cDNA library and by comparing their frequency with that in an immature cDNA library. The second strategy focused on the detailed analysis of the expression of a specific group of genes coding for the class I LEA proteins, the Em genes, and analysis of their promoter. Finally we evaluated the expression of a number of LEA gene in various regulatory mutants, including abi3, lec1 and abi5.Altogether, our results illustrate the complexity of expression patterns and the interaction of various factors to define several distinct regulatory pathways.