Exogenous Application of RNAi-Inducing Double-Stranded RNA Inhibits Aphid-Mediated Transmission of a Plant Virus
Exogenous Application of RNAi-Inducing Double-Stranded RNA Inhibits Aphid-Mediated Transmission of a Plant Virus
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DOI：
10.3389/fpls.2019.00265
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发表时间：
2019-03-15
影响因子：
5.6
通讯作者：
Mitter, Neena
中科院分区：
文献类型：
--
作者：
Worrall, Elizabeth A.;Bravo-Cazar, Ana;Mitter, Neena
植物病毒很难控制，它们降低了农作物的质量和产量，从而威胁到全球粮食安全。一种新的方法，使用局部应用双链RNA(DsRNA)来诱导抗病毒RNA干扰，已被证明在机械接种后有效地防止了一系列植物中的病毒感染。在本研究中，局部应用dsRNA对机械接种和蚜介体接种马铃薯Y病毒菜豆普通花叶病毒(BCMV)有效。局部应用针对马铃薯Y病毒核包涵体b(NIB)蛋白编码区(BCMVNIb-dsRNA)或外壳蛋白(CP)编码区(BCMVCP-dsRNA)的dsRNA可以保护本田烟草和豌豆(Vigna Unguulata)植物免受BCMV的机械接种。选择BCMVCP-dsRNA进行了后续的传毒实验。将BCMVCP-dsRNA负载到层状双氢氧化物纳米粒子上，形成BCMVCP-BioClay，这是一种比裸露dsRNA更稳定的制剂。BCMVCP-BioClay在保护植物抵御桃蚜传播BCMV方面取得了成功。用BCMVCP-BioClay喷施离体叶后5天，再用BCMVCP-BioClay喷雾处理，可保护叶片免受BCMV的侵染。重要的是，BCMVCP-BioClay在暴露于含有致病力的蚜虫前5天喷洒未受BCMVCP-BioClay感染的本氏木豆和豌豆植株，可保护这两种植物免受BCMV感染。这项研究表明，使用BioClay局部应用dsRNA可以保护植物免受蚜虫介导的病毒传播，这是发展这一方法在作物保护中的实际应用的重要的第一步。
Plant viruses are difficult to control, and they decrease both the quality and yield of crops, thus threatening global food security. A new approach that uses topical application of double-stranded RNA (dsRNA) to induce antiviral RNA-interference has been shown to be effective at preventing virus infection in a range of plants following mechanical inoculation. In this study, topical application of dsRNA was effective against mechanical inoculation and aphid-mediated inoculation with the potyvirus bean common mosaic virus (BCMV). Topical application of dsRNAs targeting either the coding region of the potyviral nuclear inclusion b (Nib) protein (BCMVNIb-dsRNA) or the coat protein (CP) coding region (BCMVCP-dsRNA) protected Nicotiana benthamiana and cowpea (Vigna unguiculata) plants against mechanical inoculation with BCMV. BCMVCP-dsRNA was selected for subsequent aphid transmission experiments. BCMVCP-dsRNA was loaded onto layered double hydroxide nanoparticles to form BCMVCP-BioClay which is a more stable formulation for delivering dsRNA than naked dsRNA. BCMVCP-BioClay was shown to be successful in protecting plants against BCMV transmission by the aphid Myzus persicae. Spraying detached N. benthamiana leaves with BCMVCP-BioClay 5 days prior to exposure to viruliferous aphids protected the leaves from infection by BCMV. Importantly, spraying of intact N. benthamiana and cowpea plants with BCMVCP-BioClay 5 days prior to exposure to viruliferous aphids protected plants of both species from BCMV infection. This study demonstrates that topical application of dsRNA using BioClay protects plants from aphid-mediated virus transmission, which is an important first step toward developing practical application of this approach in crop protection.