INCREASED PROTEIN SECRETION AND ADHERENCE TO HELA-CELLS BY SHIGELLA SPP FOLLOWING GROWTH IN THE PRESENCE OF BILE-SALTS
INCREASED PROTEIN SECRETION AND ADHERENCE TO HELA-CELLS BY SHIGELLA SPP FOLLOWING GROWTH IN THE PRESENCE OF BILE-SALTS
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DOI：
10.1128/iai.63.9.3642-3648.1995
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发表时间：
1995-09-01
影响因子：
3.1
通讯作者：
PAYNE, SM
中科院分区：
文献类型：
--
作者：
POPE, LM;REED, KE;PAYNE, SM
志贺氏菌属的生长。胆汁盐脱氧胆酸盐或鹅脱氧胆酸盐的存在增强了HeLa细胞的细菌侵袭，而在其他结构相似的胆盐或去垢剂存在的情况下，生长几乎没有影响或没有影响。当细菌在低温下暴露于脱氧胆酸盐或在生长培养基中添加氯霉素时，没有观察到脱氧胆酸盐增强的侵袭，这表明细菌生长和蛋白质合成是必需的。侵袭的增加与完整志贺氏菌毒力质粒的存在相关，并且与一组蛋白质（包括 Ipa 蛋白质）向外膜和生长培养基的分泌增加相关。胆汁盐引起的侵袭增加似乎是由于粘附性增加所致。增强的粘附性是志贺氏菌属特有的，因为测试的肠侵袭性大肠杆菌菌株没有表现出响应胆汁盐生长的效果。
Growth of Shigella spp. in the presence of the bile salt deoxycholate or chenodeoxycholate enhanced the bacterial invasion of HeLa cells, Growth in the presence of other structurally similar bile salts or detergents had little or no effect. Deoxycholate-enhanced invasion was not observed when bacteria were exposed to deoxycholate at low temperatures or when chloramphenicol was added to the growth medium, indicating that bacterial growth and protein synthesis are required. Increased invasion is associated with the presence of an intact Shigella virulence plasmid and is correlated with increased secretion of a set of proteins, including the Ipa proteins, to the outer membrane and into the growth medium. The increased invasion induced by the bile salts appears to be due to increased adherence. The enhanced adherence was specific to Shigella spp,, since the enteroinvasive Escherichia coli strains tested did not exhibit the effect in response to growth in bile salts.