Mapping of antigenic epitopes on the alpha 1 subunit of the inhibitory glycine receptor.
Mapping of antigenic epitopes on the alpha 1 subunit of the inhibitory glycine receptor.
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抑制性甘氨酸受体 α1 亚基上的抗原表位作图。
DOI：
10.1021/bi00215a007
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发表时间：
1991
期刊：
影响因子：
2.9
通讯作者：
Heinrich Betz
中科院分区：
文献类型：
--
作者：
Stephan Schroeder;Werner Hoch;C. Becker;Gabriele Grenningloh;Heinrich Betz
抑制性甘氨酸受体（GlyR）是一种配体门控的氯通道蛋白，存在于脊椎动物中枢神经系统的发育调节亚型中。针对GlyR的单克隆抗体（mab）通过识别这些受体亚型中不同的α亚基变体来区分新生儿和成人GlyR蛋白。在这里，细菌表达的大鼠GlyR α 1亚基融合蛋白被用于定位该蛋白n端105个氨基酸的主要抗原表位。合成的多肽可以进一步精细定位两个单抗结合域。MAb 2b特异于成人α 1亚基，与1-10氨基酸对应的肽结合，而MAb 4a识别新生儿和成人GlyR亚型，与代表α 1多肽96-105残基的肽反应。这些数据定义了GlyR α亚基变体上独特和常见的抗原表位。
The inhibitory glycine receptor (GlyR) is a ligand-gated chloride channel protein that occurs in developmentally regulated isoforms in the vertebrate central nervous system. Monoclonal antibodies (mAbs) against the GlyR distinguish neonatal and adult GlyR proteins by identifying distinct alpha subunit variants within these receptor isoforms. Here, bacterially expressed fusion proteins of the rat GlyR alpha 1 subunit were used to localize the major antigenic epitopes of this protein within its N-terminal 105 amino acids. Synthetic peptides allowed further fine mapping of two mAb binding domains. MAb 2b, specific for the adult alpha 1 subunit, bound to a peptide corresponding to amino acids 1-10, whereas mAb 4a, which recognizes both neonatal and adult GlyR isoforms, reacted with a peptide representing residues 96-105 of the alpha 1 polypeptide. These data define unique and common antigenic epitopes on GlyR alpha subunit variants.
烟碱乙酰胆碱受体的跨膜形貌：免疫化学测试与基于疏水性特征的理论预测相矛盾。
影响因子：
2.9
作者：
Ratnam,M;Nguyen,DL;Rivier,J;Sargent,PB;Lindstrom,J
通讯作者：
Lindstrom,J