Isoflurane attenuates LPS-induced acute lung injury by targeting miR-155-HIF1-alpha
Isoflurane attenuates LPS-induced acute lung injury by targeting miR-155-HIF1-alpha
复制标题
异氟烷通过靶向 miR-155-HIF1-alpha 减轻 LPS 诱导的急性肺损伤。
DOI：
10.2741/4302
复制
发表时间：
2015-01-01
影响因子：
3.1
通讯作者：
Jiang, Hong
中科院分区：
文献类型：
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作者：
Hu, Rong;Zhang, Ying;Jiang, Hong
异氟醚减轻内毒素诱导的急性肺损伤（ALI）的炎症反应。本研究旨在探讨异氟醚后处理对脂多糖（LPS）诱导的急性肺损伤（ALI）的保护机制。暴露于异氟烷降低了miR-155，上调了HIF-1 α和HO-1 mRNA和蛋白。异氟醚对HIF-1 α mRNA和蛋白的影响可被miR-155过表达所抑制。此外，当LPS刺激后暴露于异氟醚时，过表达miR-155的小鼠BALF中TNF-α和IL-1 β的水平更高。相反，下调miR-155促进异氟醚对HIF-1 α表达的影响。这些结果表明，异氟烷后处理通过触发miR-155-HIF-1 α通路，导致HO-1上调，从而减轻LPS诱导的ALI和细胞损伤。
Isoflurane alleviates the inflammatory response in endotoxin-induced acute lung injury (ALI). In this study, we investigated the protective mechanism of isoflurane postconditioning in lipopolysaccharide (LPS)induced ALI. Exposure to isoflurane decreased miR-155 and upregulated HIF-1 alpha and HO-1 mRNA and protein. The effects of isoflurane on HIF-1 alpha mRNA and protein could be inhibited by overexpression of miR-155. Furthermore, mice overexpressing miR-155 had higher levels of TNF-alpha and IL-1 beta in BALF when exposed to isoflurane after LPS challenge.Conversely, downregulation of miR-155 promoted isoflurane effects on HIF-1 alpha expression. These results suggest that isoflurane posttreatment hr alleviates LPS-induced ALI and cell injury by triggering miR-155-HIF-1 alpha pathway, leading to upregulation of HO-1.