Dysbiosis of the faecal microbiota in patients with Crohn's disease and their unaffected relatives
Dysbiosis of the faecal microbiota in patients with Crohn's disease and their unaffected relatives
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DOI：
10.1136/gut.2010.223263
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发表时间：
2011-05-01
期刊：
影响因子：
24.5
通讯作者：
Vermeire, Severine
中科院分区：
文献类型：
--
作者：
Joossens, Marie;Huys, Geert;Vermeire, Severine
背景和目的克罗恩病（CD）患者普遍存在肠道菌群失调，但缺乏对这种失调的系统描述。因此，将乳糜泻患者的主要粪便微生物群组成与未受影响的对照组的主要组成进行比较。还检查了乳糜泻患者的亲属是否存在生态失调。方法以至少有3名成员患有乳糜泻的家庭为研究对象，采用变性梯度凝胶电泳（DGGE）对68名乳糜泻患者、84名未患乳糜泻的亲属和55名对照者的粪便样本进行优势菌群的群落指纹鉴定。采用BioNumerics软件和非参数统计分析（SPSS V. 17.0）对DGGE谱进行分析。观察到的优势菌群差异随后用实时PCR证实和定量。结果5种细菌在CD中表现出生态失调，即内隐Dialister (p = 0.04), Clostridium cluster XIVa (p = 0.03), Faecalibacterium prausnitzii （p = 0.03）
Background and aims A general dysbiosis of the intestinal microbiota has been established in patients with Crohn's disease (CD), but a systematic characterisation of this dysbiosis is lacking. Therefore the composition of the predominant faecal microbiota of patients with CD was studied in comparison with the predominant composition in unaffected controls. Whether dysbiosis is present in relatives of patients CD was also examined.Methods Focusing on families with at least three members affected with CD, faecal samples of 68 patients with CD, 84 of their unaffected relatives and 55 matched controls were subjected to community fingerprinting of the predominant microbiota using denaturing gradient gel electrophoresis (DGGE). To analyse the DGGE profiles, BioNumerics software and non-parametric statistical analyses (SPSS V. 17.0) were used. Observed differences in the predominant microbiota were subsequently confirmed and quantified with real-time PCR.Results Five bacterial species characterised dysbiosis in CD, namely a decrease in Dialister invisus (p = 0.04), an uncharacterised species of Clostridium cluster XIVa (p = 0.03), Faecalibacterium prausnitzii (p