Comparison of the antioxidant effects of Concord grape juice flavonoids and α-tocopherol on markers of oxidative stress in healthy adults
Comparison of the antioxidant effects of Concord grape juice flavonoids and α-tocopherol on markers of oxidative stress in healthy adults
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DOI：
10.1093/ajcn/76.6.1367
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发表时间：
2002-12-01
影响因子：
7.1
通讯作者：
Jialal, I
中科院分区：
文献类型：
--
作者：
O'Byrne, DJ;Devaraj, S;Jialal, I
背景资料：康科德葡萄汁（CGJ）是一种丰富的黄酮类化合物的来源，其在体外具有比α-生育酚更大的抗氧化功效;然而，黄酮类化合物和α-生育酚在体内的功效尚未进行比较。目的：我们比较了健康成人中CGJ与α-生育酚的体内抗氧化功效。设计：受试者被随机分配接受400 IU RRR-alpha-生育酚/d（n = 17）或10 mL CGJ。kg（-1）。d（-1）（n = 15），2 wk。作为抗氧化状态和氧化应激的标志物，在补充前和补充后测量血清氧自由基吸收能力、血浆蛋白羰基、尿F-2-异前列腺素和LDL对离体氧化的抵抗力。血浆总酚和结合酚分别增加17%（P < 0.01）和22%（P < 0.001）。两组的血浆三酰甘油均发生显著变化，但浓度均在正常范围内。CGJ补充与显著高于α-生育酚补充的三酰甘油相关。两种补充方案均能显著提高血清氧自由基吸收能力（P < 0.001）和LDL延迟时间（P < 0.001），显著降低LDL氧化速率（P < 0.01），但效果无显著性差异。补充CGJ后，天然血浆中蛋白质羰基浓度下降20%，与补充α-生育酚后相比，差异有统计学意义（P < 0.05）。kg（-1）。d（-1）增加血清抗氧化能力，保护LDL免受氧化，其程度与400 IU α-生育酚/d相似，但降低天然血浆蛋白氧化的程度显著高于α-生育酚。CGJ类黄酮是有效的抗氧化剂，可以防止氧化应激，降低自由基损伤和慢性疾病的风险。
Background: Concord grape juice (CGJ) is a rich source of flavonoids, which have greater antioxidant efficacy in vitro than does alpha-tocopherol; however, the efficacies of flavonoids and alpha-tocopherol in vivo have not been compared.Objective: We compared the in vivo antioxidant efficacy of CGJ with that of alpha-tocopherol in healthy adults.Design: Subjects were randomly assigned to receive either 400 IU RRR-alpha-tocopherol/d (n = 17) or 10 mL CGJ . kg(-1) . d(-1) (n = 15) for 2 wk. Serum oxygen radical absorbance capacity, plasma protein carbonyls, urinary F-2-isoprostanes, and resistance of LDL to ex vivo oxidation were measured before and after supplementation as markers of antioxidant status and oxidative stress.Results: After supplementation, plasma alpha-tocopherol increased 92% in subjects who received a-tocopherol (P < 0.001); plasma total and conjugated phenols increased 17% (P < 0.01) and 22% (P < 0.001), respectively, in subjects who received CGJ. There was a significant change in plasma triacylglycerols in both groups, but the concentrations were within the normal range. CGJ supplementation was associated with significantly higher triacylglycerols than was alpha-tocopherol supplementation. Both supplementation regimens significantly increased serum oxygen radical absorbance capacity (P < 0.001) and LDL lag time (P < 0.001) and significantly decreased the LDL oxidation rate (P < 0.01), with no significant difference in effectiveness. Protein carbonyl concentrations in native plasma decreased 20% after CGJ supplementation, which was a significantly different response than that after alpha-tocopherol supplementation (P < 0.05).Conclusions: In healthy adults, 10 mL CGJ . kg(-1) . d(-1) increased serum antioxidant capacity and protected LDL against oxidation to an extent similar to that obtained with 400 IU alpha-tocopherol/d but decreased native plasma protein oxidation significantly more than did alpha-tocopherol. CGJ flavonoids are potent antioxidants that may protect against oxidative stress and reduce the risk of free radical damage and chronic diseases.