Electrostatic repulsion hydrophilic interaction chromatography for isocratic separation of charged solutes and selective isolation of phosphopeptides
Electrostatic repulsion hydrophilic interaction chromatography for isocratic separation of charged solutes and selective isolation of phosphopeptides
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DOI：
10.1021/ac070997p
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发表时间：
2008-01-01
影响因子：
7.4
通讯作者：
Alpert, Andrew J.
中科院分区：
文献类型：
--
作者：
Alpert, Andrew J.
如果离子交换柱主要用有机移动的相洗脱，则溶质可以通过亲水相互作用保留，即使它们具有与固定相相同的电荷。这种组合被称为静电排斥-亲水相互作用色谱（ERLIC）。对于电荷差异很大的溶质的混合物，可以利用排斥效应来选择性地拮抗通常被最好地保留的溶质的保留。这允许通常需要梯度的混合物的等度分辨率，包括肽、氨基酸和核苷酸。ERLIC可方便地分离无法通过其他方法分离的高电荷肽。此外，可以从胰蛋白酶消化物中选择性地分离磷酸肽。
If an ion-exchange column is eluted with a predominantly organic mobile phase, then solutes can be retained through hydrophilic interaction even if they have the same charge as the stationary phase. This combination is termed electrostatic repulsion-hydrophilic interaction chromatography (ERLIC). With mixtures of solutes that differ greatly in charge, repulsion effects can be exploited to selectively antagonize the retention of the solutes that normally would be the best retained. This permits the isocratic resolution of mixtures that normally require gradients, including peptides, amino acids, and nucleotides. ERLIC affords convenient separations of highly charged peptides that cannot readily be resolved by other means. In addition, phosphopeptides can be isolated selectively from a tryptic digest.