Temporally controlled site-specific mutagenesis in the germ cell lineage of the mouse testis
Temporally controlled site-specific mutagenesis in the germ cell lineage of the mouse testis
复制标题
DOI：
10.1095/biolreprod.102.005801
复制
发表时间：
2003-02-01
影响因子：
3.6
通讯作者：
Chambon, P
中科院分区：
文献类型：
--
作者：
Weber, P;Schuler, M;Chambon, P
我们获得了 PrP-Cre-ERT 转基因小鼠系 (28.8)，该小鼠系在含有小鼠朊病毒蛋白 (PrP) 启动子的基因组片段的控制下，在睾丸中选择性表达他莫昔芬诱导型 Cre-ERT 重组酶。 Cre-ERT 在精原细胞和精母细胞中表达，但在支持细胞和间质细胞中不表达。我们还建立了 PrP-L-EGFP-L 转基因小鼠，其含有 LoxP 侧翼的增强型绿色荧光蛋白 (EGFP) Cre 报告盒，受相同的包含 PrP 启动子的基因组片段控制，该片段在大脑和睾丸中表现出显着的 EGFP 表达。使用 PrP-L-EGFP-L 以及其他 Cre 报告小鼠，我们证明他莫昔芬给药有效且选择性地诱导生殖细胞谱系中 Cre 介导的重组。建立的 PrP-Cre-ERT 系应该为研究涉及精子发生的生殖细胞表达基因的功能提供有价值的工具。
We have obtained a PrP-Cre-ERT transgenic mouse line (28.8) that selectively expresses in testis the tamoxifen-inducible Cre-ERT recombinase under the control of a mouse Prion protein (PrP) promoter-containing genomic fragment. Cre-ERT is expressed in spermatogonia and spermatocytes, but not in Sertoli and Leydig cells. We also established reporter PrP-L-EGFP-L transgenic mice harboring a LoxP-flanked enhanced green fluorescent protein (EGFP) Cre reporter cassette under the control of the same PrP promoter-containing genomic fragment that exhibits prominent EGFP expression in brain and testis. Using the PrP-L-EGFP-L as well as other Cre-reporter mice, we demonstrate that tamoxifen administration efficiently and selectively induces Cre-mediated recombination in the germ cell lineage. The established PrP-Cre-ERT line should provide a valuable tool for studying functions of germ cell-expressed genes involved in spermatogenesis.