The structural basis of interlamellar cohesion in the intervertebral disc wall
The structural basis of interlamellar cohesion in the intervertebral disc wall
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DOI：
10.1111/j.1469-7580.2006.00536.x
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发表时间：
2006-03-01
影响因子：
2.4
通讯作者：
Broom, ND
中科院分区：
文献类型：
--
作者：
Pezowicz, CA;Robertson, PA;Broom, ND
本研究的目的是研究构成椎间盘环空的同心片之间产生内聚力的结构机制。切片厚度为50-60 μ m，采用精心选择的切割平面，将纤维成分作为面内和横截面阵列在交替的薄片中结合。然后，在完全水合状态下，这些部分在平面内纤维方向（径向）和（切向）上进行微拉伸拉伸。采用高分辨率诺玛斯基干涉对比光学显微镜同时观察切片，研究结构响应。在保持恒定的径向拉伸状态下，固定额外的大块环空样品，以研究在更能代表完整椎间盘壁的状态下发生层间分离的可能性。该研究提供了形成椎间盘壁内聚力的结构结构的详细图片，揭示了椎间盘壁内复杂的相互连接关系的层次结构，这是以前没有描述过的。重要的是，由于我们的实验方法提供了在完全水化状态下板间连接点对变形的响应的高分辨率视图，因此它是一种潜在的有用方法，用于研究由早期退变和全椎间盘创伤引起的连接点凝聚力的细微变化。
The purpose of this study was to investigate the structural mechanisms that create cohesion between the concentric lamellae comprising the disc annulus. Sections, 50-60 mu m thick, were obtained using a carefully chosen cutting plane that incorporated the fibrous component in alternating lamellae as in-plane and cross-sectioned arrays. These sections were then subjected to microtensile stretching both across (radial) and along (tangential) the in-plane fibre direction, in their fully hydrated state. Structural responses were studied by simultaneously viewing the sections using high-resolution Nomarski interference contrast light microscopy. Additional bulk samples of annulus were fixed while held in a constant, radially stretched state in order to investigate the potential for interlamellar separation to occur in a state more representative of the intact disc wall. The study has provided a detailed picture of the structural architecture creating disc wall cohesion, revealing a complex hierarchy of interconnecting relationships within the disc wall, not previously described. Importantly, because our experimental approach offers a high-resolution view of the response of the interlamellar junction to deformation in its fully hydrated condition, it is a potentially useful method for investigating subtle changes in junction cohesion resulting from both early degeneration and whole-disc trauma.