Identifying factors inducing trophozoite differentiation into hypnospores in Perkinsus species
Identifying factors inducing trophozoite differentiation into hypnospores in Perkinsus species
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DOI：
10.1016/j.ejop.2012.07.004
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发表时间：
2013-05-01
影响因子：
2.9
通讯作者：
La Peyre, Jerome F.
中科院分区：
文献类型：
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作者：
Casas, Sandra M.;La Peyre, Jerome F.
当在雷氏硫乙醇酸盐流体培养基（RFTM）中孵育时，帕金虫属的滋养体在宿主组织中容易分化成休眠孢子。相比之下，休眠孢子很少在体内观察到，当报道它们与垂死的宿主有关时。本研究的目的是确定什么改变的环境条件触发帕金虫滋养体分化成休眠孢子。在研究的第一部分中，培养的P.chesapeaki滋养体暴露于低氧、酸性pH、增加的营养水平、热休克或渗透休克条件下，并测量休眠孢子密度。酸性pH值，降低氧气，或增加营养水平显着增加P.切萨皮克休眠孢子的形成。在研究的第二部分中，将奥氏毕赤酵母和海洋毕赤酵母滋养体暴露于酸性pH、低氧或增加营养水平，导致奥氏毕赤酵母而不是海洋毕赤酵母中形成休眠孢子。本研究表明，环境条件的变化与腐烂组织或RFTM孵育的预期变化一致，可诱导滋养体分化。培养的滋养体的反应不同物种之间和同一物种的分离株。(C)2012 Elsevier GmbH. All rights reserved.
Trophozoites of species of Perkinsus in host tissues readily differentiate into hypnospores when incubated in Ray's fluid thioglycollate medium (RFTM). In contrast, hypnospores have rarely been observed in vivo, and when reported they have been associated with dying hosts. The objective of this study was to determine what altered environmental conditions trigger the differentiation of Perkinsus trophozoites into hypnospores. In the first part of the study, cultured P. chesapeaki trophozoites were exposed to lowered oxygen, acidic pH, increased nutrient levels, heat shock, or osmotic shock conditions, and hypnospore density was measured. Acidic pH, lowered oxygen, or increased nutrient levels significantly increased P. chesapeaki hypnospore formation. In the second part of the study, P. olseni and P. marinus trophozoites were exposed to acidic pH, lowered oxygen, or increased nutrient levels resulting in hypnospore formation in P. olseni but not P. marinus. This study demonstrated that changes in environmental conditions consistent with changes expected in decaying tissues or with RFTM incubation induce trophozoite differentiation. The response of the cultured trophozoites varied between species and between isolates of the same species. (C) 2012 Elsevier GmbH. All rights reserved.