Single Molecule Atomic Force Microscopy Studies of Photosensitized Singlet Oxygen Behavior on a DNA Origami Template
Single Molecule Atomic Force Microscopy Studies of Photosensitized Singlet Oxygen Behavior on a DNA Origami Template
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DOI：
10.1021/nn102701f
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发表时间：
2010-12-01
期刊：
影响因子：
17.1
通讯作者：
Gothelf, Kurt V.
中科院分区：
文献类型：
--
作者：
Helmig, Sarah;Rotaru, Alexandru;Gothelf, Kurt V.
DNA“折纸”是将长的单链DNA序列（支架链）通过数百个短的合成寡核苷酸（钉合链）折叠成平行排列的螺旋，是形成先进的自组装DNA结构的高效方法。由于分子和各种材料可以缀合到每个短钉链，折纸方法提供了将分子和材料排列在结构化表面上明确定义的位置的独特可能性。在这里，我们结合联合收割机的AFM和DNA纳米结构的光的作用，研究单线态氧的生产从一个单一的光敏剂分子共轭选定的DNA折纸结构上的折纸钉链。我们证明了距离依赖性氧化的有机部分纳入DNA折纸上的特定位置由单线态氧产生的一个单一的光敏剂位于每个折纸的中心。
DNA origami, the folding of a long single-stranded DNA sequence (scaffold strand) by hundreds of short synthetic oligonudeotides (staple strands) into parallel aligned helices, is a highly efficient method to form advanced self-assembled DNA-architectures. Since molecules and various materials can be conjugated to each of the short staple strands, the origami method offers a unique possibility of arranging molecules and materials in well-defined positions on a structured surface. Here we combine the action of light with AFM and DNA nanostructures to study the production of singlet oxygen from a single photosensitizer molecule conjugated to a selected DNA origami staple strand on an origami structure. We demonstrate a distance-dependent oxidation of organic moieties incorporated in specific positions on DNA origami by singlet oxygen produced from a single photosensitizer located at the center of each origami.