COMPARISON OF IMMUNOBLOTTING AND INDIRECT ENZYME-LINKED IMMUNOSORBENT-ASSAY USING DIFFERENT ANTIGEN PREPARATIONS FOR DIAGNOSING EARLY LYME-DISEASE
COMPARISON OF IMMUNOBLOTTING AND INDIRECT ENZYME-LINKED IMMUNOSORBENT-ASSAY USING DIFFERENT ANTIGEN PREPARATIONS FOR DIAGNOSING EARLY LYME-DISEASE
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DOI：
10.1093/infdis/157.4.790
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发表时间：
1988-04-01
影响因子：
6.4
通讯作者：
STEERE, AC
中科院分区：
文献类型：
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作者：
GRODZICKI, RL;STEERE, AC
我们比较了免疫印迹和间接酶联免疫吸附试验(ELISA法)使用不同的抗原制剂来检测早期莱姆病患者的伯氏疏螺旋体抗体。免疫印迹法检测急性期血清阳性16例(53%)，恢复期阳性25例(83%)。在对照组中，只有3名梅毒患者和1名住院的同种异体肾移植排斥反应患者出现假阳性结果。在莱姆病患者中，IgM和IgG抗体最常与41千道尔顿(KDa)的鞭毛抗原结合，但许多患者也与其他成分结合，特别是那些25、55、58或66 kDa的成分，它们的出现顺序是不同的。与间接酶联免疫吸附试验(使用超声处理的完整螺旋体或富含鞭毛的部分作为抗原制剂)相比，免疫印迹试验阳性的莱姆病患者较多，而对照组的假阳性较少。结果表明，免疫印迹法诊断早期莱姆病优于间接酶联免疫吸附试验。
We compared immunoblotting and indirect enzyme-linked immunosorbent assay (ELISA) using different antigen preparations to test for antibody to Borrelia burgdorferi in patients with early Lyme disease. With immunoblotting, 16 (53%) of 30 patients had positive tests in acute-phase sera and 25 (83%) had them in convalescent-phase sera. Among 64 controls, false-positive results were obtained in only three individuals with syphilis and in one hospitalized patient with renal allograft rejection. Among the patients with Lyme disease, both IgM and IgG antibodies most commonly bound to the 41-kilodalton (kDa) flagellar antigen, but many patients had binding to other components, particularly those of 25, 55, 58, or 66 kDa, and the order of their appearance was variable. Compared with indirect ELISA (using sonicated whole spirochetes or a flagellin-enriched fraction as the antigen preparation), more patients with Lyme disease had positive tests by immunoblotting, and fewer control subjects had false-positive results. Our results indicate that immunoblotting is superior to indirect ELISA for diagnosing early Lyme disease.