Soluble skin test antigen of varicella-zoster virus prepared from the fluid of infected cultures.
Soluble skin test antigen of varicella-zoster virus prepared from the fluid of infected cultures.
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从感染培养物的液体中制备水痘带状疱疹病毒的可溶性皮试抗原。
DOI：
10.1093/infdis/143.5.684
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
T. Yazaki
中科院分区：
文献类型：
--
作者：
Y. Asano;K. Shiraki;M. Takahashi;H. Nagai;T. Ozaki;T. Yazaki
利用水痘-带状疱疹病毒感染的人二倍体细胞培养液研制了水痘病毒可溶性皮试抗原。将培养液高速离心（100，000 g，2小时）以除去病毒颗粒，并将上清液在56 ℃加热30分钟后用作可溶性皮肤试验抗原。电镜下未发现病毒粒子或核衣壳，可溶性抗原中的3 H标记DNA与病毒DNA杂交极少。经免疫沉淀、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和荧光显色，可溶性抗原中检测到多条病毒糖蛋白带。在对10名医学生和10名儿童使用从培养液或感染细胞制备的可溶性抗原和粗抗原进行的比较皮肤试验中，对可溶性抗原的皮肤反应倾向于低于对粗抗原的皮肤反应，但前者的反应与水痘病史和有无病毒中和抗体密切相关。
A soluble varicella virus antigen for skin testing was developed using culture fluid from varicella-zoster virus-infected human diploid cells. The culture fluid was centrifuged at a high speed (100,000 g for 2 hr) to remove viral particles, and the supernatant, after heating at 56 C for 30 min, was used as the soluble skin test antigen. No virion or nucleocapsid was found by electron microscopy, and very little 3 H-labeled DNA in the soluble antigen hybridized with viral DNA. By immunoprecipitation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and fluorography, several viral glycoprotein bands were detected in the soluble antigen. In a comparative skin test on 10 medical students and 10 children using soluble and crude antigens prepared from culture fluid or infected cells, the skin reaction to the soluble antigen tended to be less than that to the crude antigens, but the former reaction correlated well with a history of varicella and the presence or absence of virus-neutralizing antibodies.