Effects of Extracellular Matrix Molecules on the Growth Properties of Oligodendrocyte Progenitor Cells In Vitro
Effects of Extracellular Matrix Molecules on the Growth Properties of Oligodendrocyte Progenitor Cells In Vitro
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DOI：
10.1002/jnr.22111
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发表时间：
2009-10-01
影响因子：
4.2
通讯作者：
Xu, Xiao-Ming
中科院分区：
文献类型：
--
作者：
Hu, Jianguo;Deng, Lingxiao;Xu, Xiao-Ming
细胞外基质（ECM）是神经细胞小生境的组成部分，并调节多种细胞类型的多种功能。迄今为止，有限的信息是关于其对少突胶质细胞祖细胞（OPCs）的生长特性的生物学效应。在本研究中，我们研究了几种ECM成分的影响，即，纤连蛋白、层粘连蛋白和基质胶对从胚胎第15天大鼠脊髓分离的OPC的存活、增殖、迁移、突起延伸和纯度的影响。与非ECM底物聚-D-赖氨酸相比，所有三种ECM组分增强了OPC的这些生物学特性。不过，效果的程度却有些不同。在这些ECM中，纤连蛋白对OPCs生长特性的几乎所有方面都表现出最强的作用，这意味着该分子是OPCs体外生长的更好底物。由于其对OPCs的存活和生长促进作用，纤连蛋白可被认为是在施加OPCs的外源性递送或内源性刺激的条件下增强OPCs介导的修复的候选底物。(C)2009 Wiley-Liss，Inc.
The extracellular matrix (ECM) is a component of neural cell niches and regulates multiple functions of diverse cell types. To date, limited information is available concerning its biological effects on the growth properties of oligodendrocyte progenitor cells (OPCs). In the present study, we examined effects of several ECM components, i.e., fibronectin, laminin, and Matrigel, on the survival, proliferation, migration, process extension, and purity of OPCs isolated from embryonic day 15 rat spinal cords. All three ECM components enhanced these biological properties of the OPCs compared with a non-ECM substrate, poly-D-lysine. However, the extents of their effects were somewhat different. Among these ECMs, fibronectin showed the strongest effect on almost all aspects of the growth properties of OPCs, implying that this molecule is a better substrate for the growth of OPCs in vitro. Because of its survival-and growth-promoting effects on OPCs, fibronectin may be considered as a candidate substrate for enhancing OPC-mediated repair under conditions when exogenous delivery or endogenous stimulation of OPCs is applied. (C) 2009 Wiley-Liss, Inc.