Total Saponins from Dioscorea Septemloba Thunb Reduce Serum Uric Acid Levels in Rats with Hyperuricemia through OATP1A1 Up-regulation
Total Saponins from Dioscorea Septemloba Thunb Reduce Serum Uric Acid Levels in Rats with Hyperuricemia through OATP1A1 Up-regulation
复制标题
薯蓣总皂苷通过上调 OATP1A1 降低高尿酸血症大鼠的血清尿酸水平
DOI：
10.1007/s11596-016-1573-z
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发表时间：
2016-04-01
影响因子：
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通讯作者：
Wang, Chang-jun
中科院分区：
文献类型：
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作者：
Chen, Yan;Chen, Xiao-lin;Wang, Chang-jun
本研究的目的是评价中药鳖甲提取物葛根总皂苷（TSD）对高尿酸血症的疗效，并阐明其作用机制。采用腺嘌呤灌胃法建立大鼠高尿酸血症模型。将32只大鼠随机分为4组：模型组、丹参高、低剂量组、别嘌呤醇组。同时取8只大鼠作为正常对照。测定血清尿酸（UA）、血尿素氮（BUN）、血肌酐（Scr）和有机阴离子转运多肽1A1（OATP 1A1）水平。模型组与别嘌呤醇组、丹参组比较，各治疗组血清尿酸水平均显著降低。TSD具有与别嘌呤醇相似的作用。结果显示，各治疗组大鼠海马组织中OATP 1A1蛋白表达水平均高于模型组和正常对照组。与别嘌呤醇处理组不同，TSA处理组的胃、肝、小肠和大肠组织中OATP1A1表达水平升高。提示TSD可能通过上调OATP1A1的表达，促进UA的排泄，降低UA水平。
The aim of this study is to evaluate the efficacy of total saponins of Dioscorea (TSD), an extract of the Chinese herbal Bi Xie, on hyperuricemia and to elucidate the underlying mechanisms. The rat hyperuricemia model was established by administration of adenine. Thirty-two rats were randomly allocated into 4 groups: model group, low/high-dose TSD-treated groups, and allopurinol-treated group. Meanwhile, 8 rats were used as normal controls. Serum uric acid (UA), blood urea nitrogen (BUN), serum creatinine (Scr), and organic anion transporting polypeptide 1A1 (OATP1A1) levels were measured. Comparison between the model group and treatment (allopurinol and TSD) groups showed the serum UA levels were significantly decreased in treatment groups. TSD had similar effects to allopurinol. It was found that the OATP1A1 protein expression levels in treatment groups were higher than in model group and normal controls. And different from the allopurinol-treated groups, TSD-treated group had elevated OATP1A1 expression levels in the stomach, liver, small intestine and large intestine tissues. It was suggested that TSD may facilitate the excretion of UA and lower UA levels by up-regulating OATP1A1 expression.