PIM-1 Gene RNA Interference Induces Growth Inhibition and Apoptosis of Prostate Cancer Cells and Suppresses Tumor Progression In Vivo
PIM-1 Gene RNA Interference Induces Growth Inhibition and Apoptosis of Prostate Cancer Cells and Suppresses Tumor Progression In Vivo
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DOI：
10.1002/jso.21524
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发表时间：
2010-05-01
影响因子：
2.5
通讯作者：
Xu, Yong
中科院分区：
文献类型：
--
作者：
Zhang, Tong;Zhang, Xiaoguang;Xu, Yong
背景：本研究旨在探讨PIM-1在前列腺癌(CAP)细胞增殖和凋亡中的作用，探讨PIM-1作为前列腺癌(CAP)治疗靶点的可能性。SiRNA转染后，分析细胞形态、细胞增殖、细胞周期、细胞凋亡率。结果：PIM-1 siRNA明显抑制PIM-1的表达。在体外，沉默PIM-1基因会导致细胞形态不规则，细胞增殖减少，细胞周期进程受阻，并诱导细胞凋亡。与对照组相比，裸鼠瘤内注射PIM-1 siRNA和脂质体显著抑制了PC-3肿瘤的生长。结论：PIM-1在CAP的发生发展中起重要作用，可能成为CAP治疗的一个有趣靶点。J·奥科尔外科医生。2010：101：513-519。(C)2010年Wiley-Liss公司
Background: The goal of this study was to investigate the roles of PIM-1 in prostate cancer (CaP) cell proliferation and apoptosis, and to assess the potential of PIM-1 as a target for CaP therapy.Methods: Using RNAi technology, we knocked down the expression of PIM-1 in PC-3 cell. After siRNA transfection, cell morphology, cell proliferation, cell cycle, and apoptosis rate were analyzed. PIM-1 siRNA with Lipofectamine were injected into xenograft models to evaluate its therapeutic effect.Results: PIM-1 siRNA significantly inhibited PIM-1 expression. In vitro, silencing of the PIM-1 gene resulted in irregular cell morphology, decreased cell proliferation, inhibition of cell-cycle progression, and induction of apoptosis. Compared with control groups, intratumoral injection of PIM-1 siRNA with Lipofectamine in nude mice dramatically suppressed PC-3 tumor progression.Conclusions: PIM-1 could play important roles in the progression of CaP and may be an interesting target for CaP therapy. J. Surg. Oncol. 2010:101:513-519. (C) 2010 Wiley-Liss, Inc.