Expression of SHH signaling molecules in the developing human primary dentition.
Expression of SHH signaling molecules in the developing human primary dentition.
复制标题
SHH信号分子在发育中的人类乳牙列中的表达
DOI：
10.1186/1471-213x-13-11
复制
发表时间：
2013-04-08
影响因子：
--
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Hu X;Zhang S;Chen G;Lin C;Huang Z;Chen Y;Zhang Y
我们目前对牙齿发育的了解主要来自小鼠的研究。关于人类牙齿发生过程中基因的表达和功能知之甚少。Sonic Hedgehog（SHH）信号已被证明在小鼠多个器官的发育中起着至关重要的作用，包括牙齿。然而，SHH信号分子是否在发育中的人类胚胎牙齿中表达和功能仍然未知。我们利用基因芯片技术研究了SHH信号通路分子的表达谱。然后，我们使用原位杂交来验证和揭示一些选定的分子，包括SHH，PTC1，SMO，GLI1，GLI2和GLI3，在发育中的人胚胎牙胚的空间和时间的表达模式，并将它们与小鼠进行比较。我们发现，所有这些基因表现出相似的，但略有不同的表达模式在人类和小鼠的牙胚在帽和钟阶段。我们的研究结果表明，活跃的SHH信号在人类牙齿发育的运作，并建议在人类牙齿发育过程中的SHH信号通路的保守功能。
Our current knowledge on tooth development derives primarily from studies in mice. Very little is known about gene expression and function during human odontogenesis. Sonic Hedgehog (SHH) signaling has been demonstrated to play crucial roles in the development of multiple organs in mice, including the tooth. However, if SHH signaling molecules are expressed and function in the developing human embryonic tooth remain unknown. We conducted microarray assay to reveal the expression profile of SHH signaling pathway molecules. We then used in situ hybridization to validate and reveal spatial and temporal expression patterns of a number of selected molecules, including SHH, PTC1, SMO, GLI1, GLI2, and GLI3, in the developing human embryonic tooth germs, and compared them with that in mice. We found that all these genes exhibit similar but slightly distinct expression patterns in the human and mouse tooth germ at the cap and bell stages. Our results demonstrate the operation of active SHH signaling in the developing human tooth and suggest a conserved function of SHH signaling pathway during human odontogenesis.