[Influence on 5-fluorouracil metabolism by combination of interferon-alpha and 5-fluorouracil against human hepatocellular carcinoma xenografts].
[Influence on 5-fluorouracil metabolism by combination of interferon-alpha and 5-fluorouracil against human hepatocellular carcinoma xenografts].
复制标题
干扰素-α联合5-氟尿嘧啶对人肝癌移植瘤5-氟尿嘧啶代谢的影响
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
M. Monden
中科院分区：
文献类型：
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作者：
C. Jin;Tameyoshi Yamamoto;Masato Nakamura;I. Arai;Rho Kishin;Xu Xing;H. Nagano;K. Dono;K. Umeshita;S. Nakamori;M. Sakon;M. Monden
为了研究5-氟尿嘧啶（5- fu）和干扰素- α (ifn - α)联合使用对人肝癌细胞（HuH7, PLC/PLF/5）异种移植裸鼠的抗肿瘤活性的生化调节作用，采用5- fu和干扰素- α单独或联合使用的方法进行了实验治疗。这些药物均表现出不同程度的抗肿瘤活性。虽然ifn - α（100,000单位/只小鼠/3次/周皮下注射× 6）和5- fu （0.5 mg/只小鼠/3次/周腹腔注射）对HuH7和PLC/PLF/5均有加性抗肿瘤作用，但胸腺嘧啶合成酶（TS）、二氢嘧啶脱氢酶（DPD）、胸腺嘧啶磷酸化酶（TP）、乳清酸磷酸核糖基转移酶（OPRT）、与单独使用5-FU或ifn - α治疗的肿瘤相比，5-FU/ ifn - α联合治疗的肿瘤对尿苷磷酸化酶（UP）和尿苷激酶（UK）没有显著影响。这表明5-FU和ifn - α联合抗肿瘤活性对两种人肝癌细胞系5-FU代谢无显著影响。
To investigate the effect of biochemical modulation on antitumor activity shown by the combination of 5-Fluorouracil (5-FU) and interferon-alpha (IFN-alpha), experimental therapy was performed on human hepatocellular carcinoma cell (HuH7, PLC/PLF/5) xenografts inoculated into nude mice, using 5-FU and IFN-alpha, either alone or in combination. These agents showed antitumor activity in different degrees. Although IFN-alpha, given as 100,000 units/mouse/3 times/week subcutaneously x 6, and 5-FU, given as 0.5 mg/mouse/3 times/week intraperitoneally, showed addititive antitumor effect against HuH7 and PLC/PLF/5, the activities of thymidylate synthase (TS), dihydropyrimidine dehydrogenase (DPD), thymidine phosphorylase (TP), orotate phosphoribosyltransferase (OPRT), uridine phosphorylase (UP) and uridine kinase (UK) were not significantly influenced in the tumors treated with the 5-FU/IFN-alpha combination, compared with those treated with 5-FU or IFN-alpha alone. This suggested that antitumor activity of 5-FU and IFN-alpha in combination was not significantly involved in 5-FU metabolism in two human hepatocellular carcinoma cell lines examined.