Genetically delivered antibody protects against West Nile virus.
Genetically delivered antibody protects against West Nile virus.
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基因传递的抗体可预防西尼罗河病毒。
DOI：
10.1016/j.antiviral.2007.08.010
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发表时间：
2008
影响因子：
7.6
通讯作者：
Yamshchikov,Vladimir
中科院分区：
文献类型：
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作者：
Pereboev,Alexander;Borisevich,Viktoriya;Tsuladze,George;Shakhmatov,Mikhail;Hudman,Deborah;Kazachinskaia,Elena;Razumov,Ivan;Svyatchenko,Viktor;Loktev,Valery;Yamshchikov,Vladimir
基于基因的重组抗体基因递送是一种有前途的治疗策略，其提供了许多优点，包括持续的抗体水平、更好的安全性和更低的生产成本。在此，我们描述了重组抗体Fc-9 E2的产生，所述重组抗体Fc-9 E2包含IgG 1的人Fc与源自分泌中和西尼罗病毒（WNV）的mAb的杂交瘤9 E2的单链Fv之间的融合蛋白。Fc-9 E2显示保留亲本mAb的特异性和WNV中和能力。腺病毒介导的抗体基因的体内递送导致持续的Fc-9 E2血清水平，导致动物模型中致死性WNV感染的消除。
Gene-based delivery of recombinant antibody genes is a promising therapeutic strategy offering numerous advantages including sustained antibody levels, better safety profile and lower production cost. Here we describe generation of a recombinant antibody Fc-9E2 comprising a fusion protein between human Fc of IgG1 and a single-chain Fv derived from a hybridoma 9E2 secreting a mAb neutralizing West Nile virus (WNV). Fc-9E2 was shown to retain parental mAb's specificity and WNV-neutralizing capacity. Adenovirus-mediated in vivo delivery of the antibody gene resulted in sustained Fc-9E2 serum levels leading to abrogation of lethal WNV infection in an animal model.