Effects of aromatase mutation (ArKO) on the sexual differentiation of kisspeptin neuronal numbers and their activation by same versus opposite sex urinary pheromones
Effects of aromatase mutation (ArKO) on the sexual differentiation of kisspeptin neuronal numbers and their activation by same versus opposite sex urinary pheromones
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DOI：
10.1016/j.yhbeh.2009.11.005
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发表时间：
2010-04-01
影响因子：
3.5
通讯作者：
Gonzalez-Martinez, David
中科院分区：
文献类型：
--
作者：
Bakker, Julie;Pierman, Sylvie;Gonzalez-Martinez, David
信息素已被证明诱导LH分泌的性二态反应。在这里，我们问是否在第三脑室（RP 3V）的吻肽神经元的性二型人口可以中继性二型信息从嗅觉系统的GnRH系统。此外，我们分析了芳香化酶突变（ArKO）的影响，从而对RP 3V kisspeptin神经元的数量和这些kisspeptin神经元的反应，对异性尿信息素的雌二醇的作用。暴露于男性，但不是女性的尿液气味诱导Fos蛋白在kisspeptin神经元的RP 3V的雌性野生型（WT）小鼠，这表明这些kisspeptin神经元可能是神经回路的一部分，中继信息从嗅觉脑的GnRH系统在性二态的方式。雄性信息素诱导Fos在ArKO女性kisspeptin神经元，虽然显着低于WT女性。在ArKO小鼠中，kisspeptin神经元数量的性别分化消失，即ArKO雌性小鼠RP 3V中kisspeptin免疫反应性神经元的数量与雄性小鼠一样低，而雄性ArKO小鼠的kisspeptin神经元数量略有增加。这些结果表明，野生型小鼠kisspeptin神经元数量的性别差异反映了雌激素的组织作用。相比之下，男性尿信息素激活WT女性kisspeptin神经元的能力可能不依赖于雌二醇的组织作用，因为ArKO女性仍然表现出一些Fos/kisspeptin共激活。(C)2009年由Elsevier Inc.出版
Pheromones have been shown to induce sexually dimorphic responses in LH secretion. Here we asked whether the sexually dimorphic population of kisspeptin neurons in the rostral periventricular area of the third ventricle (RP3V) could relay sexually dimorphic information from the olfactory systems to the GnRH system. Furthermore, we analyzed the effects of aromatase mutation (ArKO) and thus the role of estradiol on RP3V kisspeptin neuronal numbers and on the response of these kisspeptin neurons to same- versus opposite-sex urinary pheromones. Exposure to male but not female urinary odors induced Fos protein in kisspeptin neurons in the RP3V of female wildtype (WT) mice, suggesting that these kisspeptin neurons may be part of the neural circuitry that relays information from the olfactory brain to the GnRH system in a sexually dimorphic manner. Male pheromones induced Fos in kisspeptin neurons in ArKO females, albeit significantly less compared to WT females. The sexual differentiation of kisspeptin neuronal number was lost in ArKO mice, i.e. the number of kisspeptin-immunoreactive neurons in the RP3V of ArKO females was as low as in male mice, whereas male ArKO mice had somewhat increased numbers of kisspeptin neurons. These results suggest that the sex difference in kisspeptin neuronal number in WT mice reflects an organizational action of estradiol in females. By contrast, the ability of male urinary pheromones to activate kisspeptin neurons in WT females may not depend on the organizational action of estradiol since ArKO females still showed some Fos/kisspeptin co-activation. (C) 2009 Published by Elsevier Inc.