Formulation and optimization of mitochondria-targeted polymeric nanoparticles.
Formulation and optimization of mitochondria-targeted polymeric nanoparticles.
复制标题
线粒体靶向聚合物纳米颗粒的配制和优化。
DOI：
10.1007/978-1-4939-2288-8_8
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Dhar,Shanta
中科院分区：
文献类型：
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作者：
Marrache,Sean;Pathak,RakeshK;Dhar,Shanta
将治疗剂靶向递送至细胞的线粒体而不改变药物性质可以是治疗各种与脑功能障碍相关的疾病的重要技术。在此，我们描述了一个详细的协议，用于合成和表征的功能化聚合物，以建立靶向纳米粒子（NP）。嵌段聚合物用亲脂性三苯基鳞（TPP）阳离子修饰，用于负载有效载荷的聚合物NP的线粒体运输。基于TPP的亲脂性阳离子具有穿过线粒体膜的能力。合成了聚（dl-丙交酯-co-乙交酯）-b-聚乙二醇-TPP和聚（dl-丙交酯-co-乙交酯）-b-聚乙二醇非靶向嵌段共聚物，并制备了它们的纳米粒。采用纳米沉淀法结合聚合物共混技术，以获得合适的尺寸和电荷的NPs有效的线粒体运输。
Targeted delivery of therapeutics to the mitochondria of cells without alteration of drug properties can be a vital technique in the treatment of a variety of mitochondrial-dysfunction-related diseases. Herein, we describe a detailed protocol for synthesis and characterization of a functionalized polymer to build mitochondria-targeted nanoparticles (NPs). The block polymer was decorated with a lipophilic triphenylphosphonium (TPP) cation for mitochondrial trafficking of payload-loaded polymeric NPs. TPP-based lipophilic cations have the ability to cross the mitochondrial membrane. A mitochondria-targeted block copolymer poly(dl-lactide-co-glycolide)-b-polyethylene glycol-TPP and a nontargeted poly(dl-lactide-co-glycolide)-b-polyethylene glycol polymer were synthesized and their NPs were prepared. A nanoprecipitation method combined with polymer blending technology was adopted in order to get suitable size and charged NPs for efficient mitochondrial trafficking.