ALBA proteins confer thermotolerance through stabilizing HSF messenger RNAs in cytoplasmic granules
ALBA proteins confer thermotolerance through stabilizing HSF messenger RNAs in cytoplasmic granules
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DOI：
10.1038/s41477-022-01175-1
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发表时间：
2022-07-11
期刊：
影响因子：
18
通讯作者：
Qian, Weiqiang
中科院分区：
文献类型：
--
作者：
Tong, Jinjin;Ren, Zhitong;Qian, Weiqiang
高温是影响植物生长和适合度的主要环境胁迫之一。热应激转录因子（HSFs）在调节热应答基因的表达中起着重要作用。然而，HSFs是如何调控的仍然不清楚。在这里，我们表明，ALBA 4，ALBA 5和ALBA 6，其中相分离成应力颗粒（SG）和加工体（PB）在热胁迫下，直接结合选定的信使RNA，包括HSF mRNA，并将它们招募到SG和PB中，以保护它们免受热胁迫下的降解。alba456三重突变体，而不是单双突变体，显示多效性发育缺陷和热应激超敏反应。XRN 4（一种细胞质5 '至3 '核糖核酸外切酶）中的突变可以拯救alba 456幼苗的所观察到的发育和热敏感表型。我们的研究揭示了一个新的调节层的HSFs，其中HSFmRNA的稳定性的冗余作用的ALBA蛋白在SGs和PB的植物耐热性。热胁迫转录因子（HSFs）在响应热胁迫中发挥关键作用。这项研究揭示了一种新的调节机制，在阿拉伯曲霉ALBA蛋白稳定热应激下细胞质颗粒中的HSFs。
High temperature is one of the major environmental stresses affecting plant growth and fitness. Heat stress transcription factors (HSFs) play critical roles in regulating the expression of heat-responsive genes. However, how HSFs are regulated remains obscure. Here, we show that ALBA4, ALBA5 and ALBA6, which phase separate into stress granules (SGs) and processing bodies (PBs) under heat stress, directly bind selected messenger RNAs, including HSF mRNAs, and recruit them into SGs and PBs to protect them from degradation under heat stress in Arabidopsis. The alba456 triple mutants, but not single and double mutants, display pleiotropic developmental defects and hypersensitivity to heat stress. Mutations in XRN4, a cytoplasmic 5 ' to 3 ' exoribonuclease, can rescue the observed developmental and heat-sensitive phenotypes of alba456 seedlings. Our study reveals a new layer of regulation for HSFs whereby HSF mRNAs are stabilized by redundant action of ALBA proteins in SGs and PBs for plant thermotolerance.Heat stress transcription factors (HSFs) play critical roles in response to heat stress. This study reveals a new regulatory mechanism in Arabidospis by which ALBA proteins stabilize HSFs in cytoplasmic granules under heat stress.