Induction of pluripotent stem cells from fibroblast cultures
Induction of pluripotent stem cells from fibroblast cultures
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DOI：
10.1038/nprot.2007.418
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Yamanaka, Shinya
中科院分区：
文献类型：
--
作者：
Takahashi, Kazutoshi;Okita, Keisuke;Yamanaka, Shinya
胚胎干细胞的临床应用面临胚胎使用和移植后组织排斥等困难。解决这些问题的一种方法是直接从体细胞中产生多能干细胞。体细胞可以通过将细胞核注入去核卵母细胞或与胚胎干细胞融合，重新编程为胚胎样状态。然而，人们对这些过程背后的机制知之甚少。我们最近表明，四种转录因子的组合可以直接从小鼠成纤维细胞培养中产生es样多能干细胞。这些细胞被称为诱导多能干细胞（iPS），可以分化成三个胚层，并用于嵌合小鼠。在这里，我们描述了iPS细胞生成的详细方法和技巧。
Clinical application of embryonic stem (ES) cells faces difficulties regarding use of embryos, as well as tissue rejection after implantation. One way to circumvent these issues is to generate pluripotent stem cells directly from somatic cells. Somatic cells can be reprogrammed to an embryonic-like state by the injection of a nucleus into an enucleated oocyte or by fusion with ES cells. However, little is known about the mechanisms underlying these processes. We have recently shown that the combination of four transcription factors can generate ES-like pluripotent stem cells directly from mouse fibroblast cultures. The cells, named induced pluripotent stem (iPS) cells, can be differentiated into three germ layers and committed to chimeric mice. Here we describe detailed methods and tips for the generation of iPS cells.