Nongenomic action of an insect steroid hormone in steroid-induced programmed cell death
Nongenomic action of an insect steroid hormone in steroid-induced programmed cell death
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DOI：
10.1016/j.mce.2006.08.005
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发表时间：
2007-01-15
影响因子：
4.1
通讯作者：
Sakurai, Sho
中科院分区：
文献类型：
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作者：
Iga, Masatoshi;Iwami, Masafumi;Sakurai, Sho
家蚕丝腺的程序性细胞死亡（PCD）是由昆虫甾体激素20-羟基蜕皮激素（20 E）触发的，并依次通过细胞皱缩、核浓缩、DNA断裂、核断裂和凋亡小体形成而进行。蛋白质合成抑制剂，放线菌酮（CHX，2毫米）诱导的细胞死亡，表现出只有核和DNA片段。当单独添加时，0.2mM CHX的浓度在诱导细胞死亡方面是无效的，但是在20 E的存在下，导致与由2 mM CHX诱导的细胞死亡类似的细胞死亡，并伴随有核浓缩。由于2和0.2 mM的CHX抑制蛋白质合成相同，DNA和核碎片似乎是由20 E的非基因组作用介导的。此外，我们还发现Ca ~（2+）-PKC-caspase-3样蛋白酶通路可能参与了非基因组作用。这些数据表明，20 E诱导的PCD是通过整合基因组和非基因组的行动。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
Programmed cell death (PCD) of the silkworm silk glands is triggered by the insect steroid hormone, 20-hydroxyecdysone (20E), and proceeds sequentially through cell shrinkage, nuclear condensation, DNA fragmentation, nuclear fragmentation and apoptotic body formation. A protein synthesis inhibitor, cycloheximide (CHX, 2 mM) induced a cell death that exhibited only nuclear and DNA fragmentation. A concentration of 0.2 mM CHX was ineffective at inducing the cell death when added alone, but in the presence of 20E, a cell death similar to that induced by 2 mM CHX was resulted with accompanying nuclear condensation. Since 2 and 0.2 mM CHX inhibited protein synthesis equally, the DNA and nuclear fragmentation appear to be mediated by a nongenomic action of 20E. In addition, we show a possible involvement of Ca2+-PKC-caspase-3 like protease pathway in the nongenomic action. The data suggest that 20E-induced PCD is accomplished through the integration of genomic and nongenomic actions. (c) 2006 Elsevier Ireland Ltd. All rights reserved.