Efficient and Cost-Effective Extraction of Genomic DNA From Formalin-Fixed and Paraffin-Embedded Tissues
Efficient and Cost-Effective Extraction of Genomic DNA From Formalin-Fixed and Paraffin-Embedded Tissues
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DOI：
10.1177/0300985810380399
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发表时间：
2011-07
影响因子：
2.4
通讯作者：
A. Weiss;N. Delcour;Axel Meyer;R. Klopfleisch
中科院分区：
文献类型：
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作者：
A. Weiss;N. Delcour;Axel Meyer;R. Klopfleisch
诊断和调查分子病理学往往不得不求助于提取DNA从福尔马林固定和石蜡包埋的组织样本。尽管有许多不同的方案报道了这类材料，提取足够数量的完整DNA仍然是一个挑战。在这里，作者报告了一种可重复、简单、经济高效的方案，从大约10至15毫克的福尔马林固定和石蜡包埋的组织样本中产生高达140 μg的DNA，并将其与现有方案进行比较。该方案允许在多种组织中稳定扩增长度达600 bp的DNA片段。
Diagnostic and investigative molecular pathology frequently has to resort to extraction of DNA from formalin-fixed and paraffin-embedded tissue samples. Although many different protocols are reported for this type of material, extraction of sufficient amounts of intact DNA is still challenging. Here, the authors report a reproducible, simple, cost-effective, and efficient protocol that yields up to 140 μg of DNA from approximately 10 to 15 mg of formalin-fixed and paraffin-embedded tissue samples and compare it to available protocols. The protocol allows stable amplification of DNA fragments up to 600 bp in length in a wide variety of tissues.