CONTRIBUTION BY PURINES TO THE NEUROGENIC RESPONSE OF THE VAS-DEFERENS OF THE GUINEA-PIG
CONTRIBUTION BY PURINES TO THE NEUROGENIC RESPONSE OF THE VAS-DEFERENS OF THE GUINEA-PIG
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DOI：
10.1016/0014-2999(81)90600-2
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发表时间：
1981-01-01
影响因子：
5
通讯作者：
WESTFALL, DP
中科院分区：
文献类型：
--
作者：
FEDAN, JS;HOGABOOM, GK;WESTFALL, DP
特异性嘌呤受体拮抗剂，芳基叠氮氨基丙酰ATP（ANAPP 3），被用来确定是否从豚鼠输精管神经释放的嘌呤有助于神经性反应。ANAPP 3是一种光亲和标记物，拮抗体外输精管对透壁刺激的收缩反应，在化合物存在下可逆，在组织存在下光活化后不可逆。利血平预处理产生的去甲肾上腺素耗竭增强了ANAPP 3的拮抗作用。未处理组织的反应仅被α-肾上腺素受体阻滞剂哌唑嗪。在ANAPP 3和哌唑嗪的组合存在下，神经源性反应降低。ANAPP 3不影响[3 H]去甲肾上腺素预标记组织中3 H的释放。ANAPP 3优先拮抗神经源性反应的初始阶段性成分，但哌唑嗪和利血平预处理优先拮抗反应的次级更紧张性成分。化学交感神经切除术后剩余的6-羟基多巴胺预处理产生的小，残留的反应增强，而不是抑制，ANAPP 3和阿托品敏感。ATP或一种相关的嘌呤，来源于肾上腺素能神经元，在该组织中作为去甲肾上腺素的共递质。
The specific purine-receptor antagonist, arylazido aminopropionyl ATP (ANAPP3), was used to determine if purines released from nerves of the guinea pig vas deferens contribute to the neurogenic response. ANAPP3, which is a photoaffinity label, antagonized contractile reponses of the in vitro vas deferens to transmural stimulation, reversibly in the presence of the compound and irreversibly after its photoactivation in the presence of the tissues. The antagonism by ANAPP3 was augmented by depletion of norepinephrine produced by reserpine pretreatment. Responses of untreated tissues were only slightly antagonized by the .alpha.-adrenoceptor blocker prazosin. Neurogenic responses were reduced in the combined presence of ANAPP3 and prazosin. ANAPP3 did not affect the release of 3H from tissues prelabeled with [3H]norepinephrine. The initial phasic component of the neurogenic response was preferentially antagonized by ANAPP3, but the secondary more tonic component of the response was preferentially antagonized by prazosin and reserpine pretreatment. Small, residual responses remaining after chemical sympathectomy produced by 6-hydroxydopamine pretreatment were potentiated, rather than inhibited, by ANAPP3 and were sensitive to atropine. ATP or a related purine, originating from adrenergic neurons, acts as a co-transmitter with norepinephrine in this tissue.