ANTIBODIES TO RECOMBINANT-DERIVED GLYCOPROTEIN-B AFTER NATURAL HUMAN CYTOMEGALOVIRUS-INFECTION CORRELATE WITH NEUTRALIZING ACTIVITY
ANTIBODIES TO RECOMBINANT-DERIVED GLYCOPROTEIN-B AFTER NATURAL HUMAN CYTOMEGALOVIRUS-INFECTION CORRELATE WITH NEUTRALIZING ACTIVITY
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DOI：
10.1093/infdis/165.2.381
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发表时间：
1992-02-01
影响因子：
6.4
通讯作者：
WALDEYER, SL
中科院分区：
文献类型：
--
作者：
MARSHALL, GS;RABALAIS, GP;WALDEYER, SL
用小扁豆凝集素柱层析法从重组腺病毒感染的细胞中部分纯化人巨细胞病毒（HCMV）糖蛋白B（gB）。 该抗原含有约130和55 kDa的特异性反应蛋白，用于研究48例自然HCMV感染后的gB抗体水平。 放射免疫沉淀（RIP）试验检测所有血清IgG抗体gB。 定量RIP显示gB抗体与中和活性之间有很强的相关性（r = 0.74，P <0.001），但gB抗体与总HCMV特异性IgG之间的相关性较弱（r = 0.36，P <0.02）。 当从20份血清标本中特异性吸收gB抗体时，中和抗体滴度降低中位数为48%（范围，0-98%）。 这些数据证实，gB抗体是HCMV中和抗体应答的重要组成部分，并支持该蛋白在亚单位疫苗开发中的作用。
Glycoprotein B (gB) of human cytomegalovirus (HCMV) was partially purified by lentil-lectin column chromatography from cells infected with an adenovirus-gB recombinant. This antigen, which contained specifically reactive proteins of approximately 130 and 55 kDa, was used to investigate gB antibody levels after natural HCMV infection in 48 individuals. All sera had IgG antibody to gB as detected by radioimmunoprecipitation (RIP) assays. Quantitative RIP showed a strong correlation between gB antibody and neutralizing activity (r = .74, P < .001) but a weak correlation between gB antibody and total HCMV-specific IgG (r = .36, P < .02). When gB antibody was specifically absorbed from 20 serum specimens, neutralizing antibody titer was reduced a median of 48% (range, 0-98%). These data confirmed that antibodies to gB are a large component of the neutralizing antibody response to HCMV and support a role for this protein in the development of subunit vaccines.