Effect of testing for IgG avidity in the diagnosis of Toxoplasma gondii infection in pregnant women:: Experience in a US reference laboratory
Effect of testing for IgG avidity in the diagnosis of Toxoplasma gondii infection in pregnant women:: Experience in a US reference laboratory
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DOI：
10.1086/319672
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发表时间：
2001-04-15
影响因子：
6.4
通讯作者：
Remington, JS
中科院分区：
文献类型：
--
作者：
Liesenfeld, O;Montoya, JG;Remington, JS
美国参考实验室评估了与弓形虫相关的 IgG 亲合力测试的有用性。欧洲研究人员报告称，高亲和力 IgG 弓形虫抗体可排除之前 3 个月内的急性感染。在这项美国研究中，回顾性地选择了从 125 名妊娠早期孕妇采集的 125 份血清样本，因为弓形虫血清学特征中的 IgM 或差异凝集 (AC/HS) 测试提示或不明确最近获得的感染。在 93 份 (74.4%) IgM ELISA 结果呈阳性或可疑的血清样本中，52 份 (55.9%) 具有高亲和力抗体，这表明感染可能是在妊娠前获得的。在 AC/HS 测试中具有急性或模棱两可结果的 87 份 (69.6%) 血清样本中，35 份 (40.2%) 具有高亲和力抗体。 40 名女性接受了螺旋霉素，以预防先天性传播，其中 7 名（17.5%）有高亲和力抗体。这些发现强调了测试妊娠前三个月获得的单一血清样本的 IgG 亲合力的价值。
The usefulness of testing for IgG avidity in association with Toxoplasma gondii was evaluated in a US reference laboratory. European investigators have reported that high-avidity IgG toxoplasma antibodies exclude acute infection in the preceding 3 months. In this US study, 125 serum samples taken from 125 pregnant women in the first trimester were chosen retrospectively, because either the IgM or differential agglutination (AC/HS) test in the Toxoplasma serologic profile suggested or was equivocal for a recently acquired infection. Of 93 (74.4%) serum samples with either positive or equivocal results in the IgM ELISA, 52 (55.9%) had high-avidity antibodies, which suggests that the infection probably was acquired before gestation. Of 87 (69.6%) serum samples with an acute or equivocal result in the AC/HS test, 35 (40.2%) had high-avidity antibodies. Forty women were given spiramycin, to prevent congenital transmission, and 7 (17.5%) had high-avidity antibodies. These findings highlight the value of testing a single serum sample obtained in the first trimester of pregnancy for IgG avidity.