Increased phenolic acid and tanshinone production and transcriptional responses of biosynthetic genes in hairy root cultures of Salvia przewalskii Maxim. treated with methyl jasmonate and salicylic acid
Increased phenolic acid and tanshinone production and transcriptional responses of biosynthetic genes in hairy root cultures of Salvia przewalskii Maxim. treated with methyl jasmonate and salicylic acid
复制标题
DOI：
10.1007/s11033-020-05899-1
复制
发表时间：
2020-10-13
影响因子：
2.8
通讯作者：
Hu, Benxiang
中科院分区：
文献类型：
--
作者：
Li, Jie;Li, Bo;Hu, Benxiang
本研究旨在揭示植物激素水杨酸(SA)和茉莉酸甲酯(MeJA)对普氏丹参毛状根生长、有效成分积累及相关基因表达的影响。将不同浓度的SA(0、25、50、100、200亩M)或MeJA(0、50、100、200、400、600亩M)添加到普氏丹参的培养基中。低浓度SA促进毛状根生长，高浓度SA抑制毛状根生长。0~400亩M MeJA促进毛状根的生长，600亩M MeJA开始抑制毛状根的生长。50亩M SA和400亩M MeJA显著提高了咖啡酸、迷迭香酸、丹酚酸B、隐丹参酮和丹参酮IIA的产量。毛状根中丹参酮的积累一般以50亩M-SA为宜。Przewalski，为期6天。400亩M MeJA可用于毛状根中酚酸的积累。Przewalski有3天的时间。丹参酮和酚酸生物合成途径中的所选基因在启发子作用下上调。为获得较高的产量和次生代谢物含量，毛状根的适宜培养剂量以50亩M SA或400亩M MeJA为宜。本研究首次提出了一种高效的丹参酮和酚酸的生产方法。普尔兹瓦尔斯基。
The purpose of this study is to reveal the impact of the plant hormone salicylic acid (SA) and methyl jasmonate (MeJA) on the growth, effective components accumulation, and related gene expression of the hairy root ofSalvia przewalskiiMaxim. Various concentrations of SA (0, 25, 50, 100, 200 mu M) or MeJA (0, 50, 100, 200, 400, 600 mu M) were added to the culture medium ofSalvia przewalskiiMaxim. Low concentrations of SA promoted the growth of hairy root, while a high concentration inhibited it. 0 to 400 mu M MeJA promoted the growth of hairy root, but 600 mu M MeJA starts to inhibit its growth. 50 mu M SA and 400 mu M MeJA significantly enhanced the production of caffeic acid, rosmarinic acid, salvianolic acid B, cryptotanshinone, and tanshinone IIA. In general, 50 mu M SA can be used to accumulate of tanshinone in hairy roots ofS. przewalskiiwith 6 days. 400 mu M MeJA can be used to accumulate of phenolic acids in hairy roots ofS. przewalskiiwith 3 days. The selected genes in the tanshinone and phenolic acid biosynthetic pathway were upregulated with elicitation. To obtain a higher yield and content of secondary metabolites, it is advisable to use 50 mu M SA or 400 mu M MeJA as the optimal doses to cultivate the hairy root ofS. przewalskii.This study provides, for the first time, an efficient tanshinone and phenolic acid production method forS. przewalskii.