ScanImage: flexible software for operating laser scanning microscopes.
ScanImage: flexible software for operating laser scanning microscopes.
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DOI：
10.1186/1475-925x-2-13
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发表时间：
2003-05-17
影响因子：
3.9
通讯作者：
Svoboda, Karel
中科院分区：
文献类型：
--
作者：
Pologruto, Thomas A.;Sabatini, Bernardo L.;Svoboda, Karel
激光扫描显微镜是分析生物标本结构和功能的有力工具。尽管存在许多商用激光扫描显微镜，但一些更有趣和更具挑战性的应用需要定制设计。定制设计的一个主要障碍是难以构建定制的数据采集硬件和编写运行激光扫描显微镜所需的复杂软件。我们描述了一种简单的，基于软件的方法来操作激光扫描显微镜，而不需要定制的数据采集硬件。通过标准的数据采集板实现激光扫描的数据采集和控制。整个信号集成和图像处理的重担都放在了计算机的CPU上。我们量化了我们的数据采集和信号调理算法在各种条件下的有效性。我们在开源软件包（ScanImage）中实现了我们的方法，并描述了它的功能。我们介绍ScanImage，软件运行灵活的激光扫描显微镜，允许轻松定制设计。
Laser scanning microscopy is a powerful tool for analyzing the structure and function of biological specimens. Although numerous commercial laser scanning microscopes exist, some of the more interesting and challenging applications demand custom design. A major impediment to custom design is the difficulty of building custom data acquisition hardware and writing the complex software required to run the laser scanning microscope. We describe a simple, software-based approach to operating a laser scanning microscope without the need for custom data acquisition hardware. Data acquisition and control of laser scanning are achieved through standard data acquisition boards. The entire burden of signal integration and image processing is placed on the CPU of the computer. We quantitate the effectiveness of our data acquisition and signal conditioning algorithm under a variety of conditions. We implement our approach in an open source software package (ScanImage) and describe its functionality. We present ScanImage, software to run a flexible laser scanning microscope that allows easy custom design.