Kainic acid upregulates uncoupling protein-2 mRNA expression in the mouse brain
Kainic acid upregulates uncoupling protein-2 mRNA expression in the mouse brain
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DOI：
10.1097/00001756-200311140-00002
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发表时间：
2003-11-14
期刊：
影响因子：
1.7
通讯作者：
Richard, D
中科院分区：
文献类型：
--
作者：
Clavel, S;Paradis, É;Richard, D
C57BL/6J和129T2SvEmsJ小鼠腹腔注射kainic酸（KA）可瞬间诱导解偶联蛋白-2 (Ucp2) mRNA在多个脑区表达，包括海马CAI亚区、梨状内核背侧和梨状皮质。在所有这些区域，通过原位杂交检测，Ucp2 m RNA的表达水平在24小时达到峰值，并在注射后72小时内恢复到基础水平。mRNA表达的增加主要在神经元中观察到，小胶质细胞仅分散表达该基因。129T2SvEmsJ小鼠对KA的神经元诱导强于C57BL/6J小鼠，这表明Ucp2在兴奋性毒性挑战和神经保护中发挥作用。
Intraperitoneal injection of kainic acid (KA) in C57BL/6J and 129T2SvEmsJ mice led to a transient induction of uncoupling protein-2 (Ucp2) mRNA expression in several brain regions, which included the CAI subfield of the hippocampus, the dorsal endopiriform nucleus and the piriform cortex in both strains. In all those regions, levels of Ucp2 m RNA expression, as determined by in situ hybridization, peaked at 24 h and returned to basal levels within 72 h post-injection. The increase in mRNA expression was mainly observed in neurons, with microglial cells displaying only scattered expression of the gene. The neuronal induction of Ucp2 in response to KA was stronger in 129T2SvEmsJ mice than in C57BL/6J, which suggests a role for Ucp2 in excitotoxic challenges and neuroprotection.