A Sex Difference in Circadian Food-Anticipatory Rhythms in Mice: Interaction With Dopamine D1 Receptor Knockout
A Sex Difference in Circadian Food-Anticipatory Rhythms in Mice: Interaction With Dopamine D1 Receptor Knockout
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DOI：
10.1037/bne0000058
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发表时间：
2015-06-01
影响因子：
1.9
通讯作者：
Mistlberger, Ralph E.
中科院分区：
文献类型：
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作者：
Michalik, Mateusz;Steele, Andrew D.;Mistlberger, Ralph E.
在小鼠和其他物种中，限制的每日喂养时间表诱导食物预期活动（FAA）的昼夜节律。驱动这些节律的昼夜节律振荡器的夹带途径和位置尚未确定。多巴胺信号传导和背侧纹状体的一个重要作用是由一个汇合的意见，包括移动的FAA节律的多巴胺受体激动剂和衰减的拮抗剂和D1受体敲除（D1 R KO）建议。多巴胺奖赏系统在结构和功能上表现出性二态性;如果FAA节律受该系统调节，那么FAA也可能是性二态性的。为了评估这一预测，连续记录雄性和雌性C57 BL/6 J小鼠的圆盘奔跑和一般活动，小鼠可随意进食，然后在光照期中间限制为每天4小时进食。与雄性小鼠相比，雌性小鼠的FAA在持续时间、总计数、峰值水平和相对于夜间活动的比率方面显著降低。为了确定这些差异是否由D1受体介导，检查了雄性和雌性纯合D1 R KO小鼠。与野生型和杂合子小鼠相比，雌性和雄性D1 R KO小鼠的FAA参数显着衰减。雌性小鼠的衰减幅度更大。这些结果证实了多巴胺D1受体在昼夜节律机制中的重要作用，通过该机制，小鼠预期每天的膳食，并且它们揭示了先前未报道的由D1 R KO放大的食物预期节律表达的性二态性。
Restricted daily feeding schedules induce circadian rhythms of food-anticipatory activity (FAA) in mice and other species. The entrainment pathway(s) and location(s) of circadian oscillators driving these rhythms have not been definitively established. An important role for dopamine signaling and the dorsal striatum is suggested by a confluence of observations, including shifting of FAA rhythms by dopamine receptor agonists and attenuation by antagonists and D1 receptor knockout (D1R KO). The dopamine reward system exhibits sexual dimorphisms in structure and function; if FAA rhythms are regulated by this system, then FAA may also be sexually dimorphic. To assess this prediction, disk running and general activity were recorded continuously in male and female C57BL/6J mice with food available ad libitum and then restricted to a 4-hr daily meal in the middle of the light period. Compared with male mice, FAA in female mice was significantly reduced in duration, total count, peak level, and ratio relative to nocturnal activity. To determine whether these differences were mediated by D1 receptors, male and female homozygous D1R KO mice were examined. Compared with wild type and heterozygous mice, female and male D1R KO mice exhibited a marked attenuation of FAA parameters. The magnitude of the attenuation was greater in female mice. These results confirm an important role for dopamine D1 receptors in the circadian mechanism by which mice anticipate a daily meal, and they reveal a previously unreported sexual dimorphism in the expression of food-anticipatory rhythms that is amplified by D1R KO.