Identification of complex, naturally occurring flavonoid glycosides in Vicia faba and Pisum sativum leaves by HPLC-DAD-ESI-MSn and the genotypic effect on their flavonoid profile
Identification of complex, naturally occurring flavonoid glycosides in Vicia faba and Pisum sativum leaves by HPLC-DAD-ESI-MSn and the genotypic effect on their flavonoid profile
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DOI：
10.1016/j.foodres.2015.02.021
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发表时间：
2015-10-01
影响因子：
8.1
通讯作者：
Schreiner, Monika
中科院分区：
文献类型：
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作者：
Neugart, Susanne;Rohn, Sascha;Schreiner, Monika
蚕豆（Vicia faba）和豌豆（Pisum sativum）是属于豆科植物的豆科植物。由于它们的黄酮类化合物具有高度多样性和相当复杂的结构，本研究的目的是鉴定不同品种的蚕豆和豌豆叶中的黄酮醇苷。在蚕豆中主要的黄酮醇苷元是山奈酚，而在豌豆中主要的黄酮醇苷元是槲皮素。此外，蚕豆叶含有槲皮素和山奈酚的单糖苷、二糖苷、三糖苷和四糖苷，主要是葡萄糖、鼠李糖和半乳糖作为糖部分。其中两个结构已被鉴定为用乙酸酰化。相比之下，在P. sativum叶中，仅三糖苷被鉴定为槐三糖苷。9的11个槲皮素和山奈酚糖苷与选定的羟基肉桂酸酰化。不同的品种，蚕豆和P. sativum提供不同的黄酮醇谱。在这里，第一次出现在豆科植物的叶子上。例如，蚕豆的夏季栽培品种显示出高浓度的山奈酚-3-O-鼠李糖阿拉伯糖苷-7-O-鼠李糖苷。然而，大蒜尤其是Salamanca品种具有最期望的类黄酮糖苷谱，涉及高浓度的槲皮素糖苷和示例性的用对香豆酸的酰化。(C)2015爱思唯尔有限公司版权所有。
Vicia faba and Pisum sativum are legumes belonging to the plant family Fabaceae. As their flavonoid profile is highly diverse with quite complex structures, the aim of this study was to identify the flavonol glycosides in the leaves of different cultivars of V. faba and P. sativum. In V. faba the main flavonol aglycon is kaempferol, whereas in P. sativum the main flavonol aglycon is quercetin. Furthermore, V. faba leaves contain mono-, di-, tri- and tetraglycosides of quercetin and kaempferol with primarily glucose, rhamnose, and galactose as sugar moieties. Two of these structures have been identified to be acylated with acetic acid. In contrast in P. sativum leaves only triglycosides were identified being sophotriosides. Nine of the 11 quercetin and kaempferol glycosides were acylated with selected hydroxycinnamic acids. Depending on cultivar, V. faba and P. sativum provided different flavonol profiles. Here, shown for the first time in legume leaves. For example, the summer cultivars of V. faba showed a high concentration of kaempferol-3-O-rhamnoarabinoside-7-O-rhamnoside. However, P. sativum with especially the cv Salamanca, had the most desired flavonoid glycoside profile referring to high concentrations of quercetin glycosides and exemplarily the acylation with p-coumaric acid. (C) 2015 Elsevier Ltd. All rights reserved.