Specific humoral reaction of hemorrhagic fever with renal syndrome (HFRS) patients in China to recombinant nucleocapsid proteins from European hantaviruses
Specific humoral reaction of hemorrhagic fever with renal syndrome (HFRS) patients in China to recombinant nucleocapsid proteins from European hantaviruses
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DOI：
10.1007/s10096-010-1134-5
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发表时间：
2011-01
影响因子：
4.5
通讯作者：
H. Xiong;Qing Li;Wen Chen;Dong-ying Liu;Jia-xin Ling;Jing Liu;Yingle Liu;Youyi Zhang;Zhan-Qiu Yang
中科院分区：
文献类型：
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作者：
H. Xiong;Qing Li;Wen Chen;Dong-ying Liu;Jia-xin Ling;Jing Liu;Yingle Liu;Youyi Zhang;Zhan-Qiu Yang
肾综合征出血热（HFRS）是东亚和欧洲的地方病。本研究旨在探讨汉坦病毒（HTNV）、多布拉瓦-贝尔格莱德病毒（DOBV）和普马拉病毒（PUUV）重组核衣壳蛋白（rNPs）与中国HFRS患者血清中抗体的反应性。收集湖北省1985-2002年肾综合征出血热（HFRS）患者急性期和恢复期血清48对，分别用6种欧洲汉坦病毒rNP作为包被抗原，采用间接IgG、伊加和IgM酶联免疫吸附试验检测。结果表明，rNP对IgG的敏感性为HTNV-rNP > DOBV-rNP > PUUV-rNP，对伊加的敏感性为DOBV-rNP > HTNV-rNP > PUUV-rNP。定量分析显示，HFRS患者急性期和恢复期血清主要表现出高水平的伊加。虽然PUUV-rNP在所有样品中对三种免疫球蛋白表现出非常弱的反应性，但三对血清出乎意料地对所有三种PUUV-rNP亚型产生强烈的交叉反应。我们首次观察到湖北省HFRS患者血清与PUUV-rNPs交叉反应和恢复期持续高水平伊加的新的流行特征，在中国也是如此。
Hemorrhagic fever with renal syndrome (HFRS) is endemic in East Asia and Europe. This study was initiated to investigate the reactivity of antibodies in sera of Chinese HFRS patients with the recombinant nucleocapsid proteins (rNPs) ofHantaan virus(HTNV),Dobrava-Belgrade virus(DOBV), andPuumala virus(PUUV), which are the prevalent hantavirus strains in Europe. Forty-eight pairs of acute and convalescent sera were collected from HFRS patients in Hubei, China (1985-2002) and tested by indirect IgG, IgA, and IgM enzyme-linked immunosorbent assays with six rNPs of European hantaviruses as coated antigens, respectively. The results showed that the sensitivity of rNPs against IgG was HTNV-rNP > DOBV-rNP > PUUV-rNP, while the sensitivity against IgA was DOBV-rNP > HTNV-rNP > PUUV-rNP. Quantitative analysis revealed both acute and convalescent sera from HFRS patients predominantly exhibit high levels of IgA. Although PUUV-rNPs showed very weak reactivity to the three kinds of immunoglobulins in all samples, three pairs of sera unexpectedly cross-reacted strongly to all three PUUV-rNP subtypes. We first observe that HFRS patients’ sera from Hubei Province show new prevalent characteristics of cross-reacting with PUUV-rNPs and continued high level of IgA in convalescent phase, as well as in China.