Healing of localized gingival recessions treated with a coronally advanced flap alone or combined with an enamel matrix derivative and a porcine acellular dermal matrix: a preclinical study
Healing of localized gingival recessions treated with a coronally advanced flap alone or combined with an enamel matrix derivative and a porcine acellular dermal matrix: a preclinical study
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DOI：
10.1007/s00784-015-1680-4
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发表时间：
2016-09-01
影响因子：
3.4
通讯作者：
Noguchi, K.
中科院分区：
文献类型：
--
作者：
Shirakata, Y.;Sculean, A.;Noguchi, K.
本研究旨在评估猪脱细胞真皮基质（PADM）加或不加牙釉质基质衍生物（EMD）对冠状进展皮瓣（CAF）治疗犬牙龈退缩缺损的影响。本文对12只犬双侧上颌牙阴唇侧行Millerⅱ类龈退缩缺损（5 mm宽，7 mm深）进行了手术治疗。斑块积累8周后，24个慢性缺陷随机分配到以下4种治疗中的一种：CAF、CAF + PADM （CAF/PADM）、CAF + EMD （CAF/EMD）和CAF + EMD + PADM （CAF/EMD/PADM）。术后10周处死动物进行组织学评估。各组均有不同程度的根系覆盖度。在CAF/PADM组和CAF/EMD/PADM组中，PADM在牙龈结缔组织中结合良好。CAF/EMD/PADM组新生骨高度显著高于CAF和CAF/PADM组。CAF/EMD组和CAF/EMD/PADM组以新牙骨质和牙周韧带样组织为主。CAF/EMD/PADM组的新牙骨质数量最多，但差异无统计学意义。在本研究的限制下，可以得出CAF/EMD/PADM治疗可以促进牙龈后退缺陷的牙周再生。目前的结果表明，EMD和PADM联合CAF可能是治疗单Miller II类牙龈衰退的一种有希望的方法。
This study aimed to evaluate the effects of a porcine acellular dermal matrix (PADM) with or without an enamel matrix derivative (EMD) on gingival recession defects treated with a coronally advanced flap (CAF) in dogs.Miller class II gingival recession defects (5 mm wide and 7 mm deep) were surgically created on the labial side of bilateral maxillary canines in 12 dogs. After 8 weeks of plaque accumulation, the 24 chronic defects were randomly assigned to one of the following 4 treatments: CAF, CAF with PADM (CAF/PADM), CAF with EMD (CAF/EMD), and CAF with EMD and PADM (CAF/EMD/PADM). The animals were sacrificed 10 weeks after surgery for histologic evaluation.In all groups, root coverage was obtained to a varying degree. PADM was well incorporated in gingival connective tissue in the CAF/PADM and in the CAF/EMD/PADM groups. The height of newly formed bone was significantly greater in the CAF/EMD/PADM group than in the CAF and CAF/PADM groups. New cementum with periodontal ligament-like tissue was predominantly found in the CAF/EMD and CAF/EMD/PADM groups. The CAF/EMD/PADM group showed the greatest amount of new cementum among the groups examined, although the difference was not statistically significant.Within the limitations of the present study, it can be concluded that CAF/EMD/PADM treatment may promote periodontal regeneration in gingival recession defects.The present results suggest that the combination of EMD and PADM in conjunction with CAF may represent a promising approach for treating single Miller class II gingival recessions.