IL-10 as an autocrine regulator of CSF secretion by monocytes: disparate effects on GM-CSF and G-CSF secretion.
IL-10 as an autocrine regulator of CSF secretion by monocytes: disparate effects on GM-CSF and G-CSF secretion.
复制标题
IL-10 作为单核细胞 CSF 分泌的自分泌调节剂：对 GM-CSF 和 G-CSF 分泌的不同影响。
作者：
S. Lenhoff;B. Sallerfors;T. Olofsson
在以前的内源性粒细胞-巨噬细胞集落刺激因子（GM-CSF）和粒细胞集落刺激因子（G-CSF）生产的研究中，我们发现不同细胞系统内和之间的分泌模式存在一些差异;例如，内皮细胞的CSF分泌不受任何主要下调因子的影响，而单核细胞的CSF分泌受几种机制的调节。在这项研究中，我们的特点是抑制单核细胞分泌CSF的因素。三种细胞因子具有抑制作用：白细胞介素（IL）-4，IL-10和IL-13。其中，IL-4和IL-10具有比IL-13更高的效力。IL-4和IL-13以相同程度影响GM-CSF和G-CSF分泌。相反，外源性添加的IL-10对GM-CSF分泌的抑制作用强于对G-CSF分泌的抑制作用。我们还发现单核细胞产生具有自分泌下调效应的IL-10，并且这种自分泌IL-10达到在大多数情况下仅GM-CSF（而非G-CSF）分泌受到显著影响的浓度。我们假设IL-10对两种CSF的单核细胞分泌的不同影响反映了它们的生理功能，GM-CSF主要是在局部隔室中工作的促炎细胞因子，而G-CSF主要作为细胞募集因子发挥作用。
In previous studies of endogenous granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF) production, we found several differences in the secretion pattern within and between different cell systems; for example, CSF secretion by endothelial cells is not affected by any major downregulatory factors, whereas monocyte CSF secretion is modulated by several mechanisms. In this study, we characterized the factors that inhibit CSF secretion by monocytes. Three cytokines have inhibitory effects: interleukin (IL)-4, IL-10, and IL-13. Among these, IL-4 and IL-10 have higher potency than IL-13. IL-4 and IL-13 affect GM-CSF and G-CSF secretion to the same extent. In contrast, exogenously added IL-10 has a stronger inhibitory effect on GM-CSF secretion than on G-CSF secretion. We also found that monocytes produce IL-10 with an autocrine downregulatory effect, and that this autocrine IL-10 reaches concentrations at which in most cases only GM-CSF (not G-CSF) secretion is significantly affected. We postulate that the disparate effect of IL-10 on monocyte secretion of the two CSFs reflects their physiological functions, with GM-CSF being mainly a proinflammatory cytokine working in the local compartment and G-CSF functioning mainly as a cell recruiting factor.