Construction of mouse A9 clones containing a single human chromosome (X/autosome translocation) via micro-cell fusion.
Construction of mouse A9 clones containing a single human chromosome (X/autosome translocation) via micro-cell fusion.
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DOI：
10.1111/j.1349-7006.1989.tb02278.x
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发表时间：
1989-03
期刊：
影响因子：
--
通讯作者：
Oshimura M
中科院分区：
文献类型：
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作者：
Koi M;Morita H;Shimizu M;Oshimura M
形成、选择和分离次黄嘌呤鸟嘌呤磷酸核糖转移酶（HGPRT）缺陷型小鼠细胞系（A9或RAG）与12种不同的含有各种X/常染色体易位的人成纤维细胞（GM细胞）之间的细胞杂交体。在这些杂交细胞中发现了包括携带HGPRT位点的X/常染色体易位在内的几条人类染色体。为了构建含有单个X/常染色体易位的A9细胞克隆，进行微细胞融合以将这些染色体从杂交体转移到A9细胞。核型分析显示，在小鼠染色体的背景下，大多数所得的微细胞杂交体仅包含用于细胞杂交的GM细胞中存在的人类X/常染色体易位。建立了含有以下常染色体片段的A9细胞亚系：1 q23 → 1 qter; 1 q12 → 1 pter; 3 p12 → 3 pter; 3q 21 →3qter; 11 q13 → 11 qter; 11 q13 → 11 pter; 11 p11 → 11 qter; 11 q23 → 11 pter; 12 q24 → 12 pter; 16 q24 → 16 pter; 17 q11 → 17 pter。
Cell hybrids between hypoxanthine guanine phosphoribosyl transferase (HGPRT)‐deficient mouse cell lines (A9 or RAG) and each of 12 different human flbroblasts (GM cells) containing various X/autosome translocations were formed, selected and isolated. Several human chromosomes including an X/autosome translocation carrying HGPRT locus were found in these hybrid cells. To construct A9 cell clones that contain a single X/autosome translocation, micro‐cell fusion was undertaken to transfer these chromosomes from the hybrids to A9 cells. Karyotype analysis revealed that most of the resulting micro‐cell hybrids contain, in a background of mouse chromosomes, only the human X/autosome translocations which were present in the GM cells used for cell hybridization. Sublines of A9 cells were established containing the following autosomal segments: 1q23→1qter; 1q12→1pter; 3p12→3pter; 3q21→3qter; 11q13→11qter; 11q13→11pter; 11p11→11qter; 11q23→11pter; 12q24→12pter; 16q24→16pter; 17q11→17pter.