Measuring microlymphatic flow using fast video microscopy -: art. no. 064016
Measuring microlymphatic flow using fast video microscopy -: art. no. 064016
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DOI：
10.1117/1.2135791
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发表时间：
2005-11-01
影响因子：
3.5
通讯作者：
Coté, GL
中科院分区：
文献类型：
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作者：
Dixon, JB;Zawieja, DC;Coté, GL
尽管淋巴功能的测量取得了进展，但人们对微淋巴管（直径类似于 100 μm）的实际流速知之甚少。在这项工作中，视频显微镜和粒子跟踪方法被采用并与超高速成像相机集成，以获得大鼠肠系膜整个淋巴收缩周期的淋巴速度测量，这是以前的系统无法测量的。为了确定系统的准确性，在假设的微淋巴流速度生理显着范围（高达 15 毫米/秒）内进行校准实验。当将实际速度与测量速度进行比较时，该系统显示出高精度，误差小于 2%。对流经 140 微米直径管道的微球进行成像，以证明系统能够通过测量颗粒速度来确定这些小容器中的流速。为了证明生物学适用性，将三只雄性 Sprague-Dawley 大鼠小肠环中的肠系膜微淋巴管外化并使用高速系统以 500 帧/秒的速率对多个收缩序列进行成像。淋巴速度随着血管壁收缩而周期性波动，范围从-1到7毫米/秒。这些速率高于标准视频显微镜（最大 3.75 毫米/秒）的速率。 (C) 2005 年光电仪器工程师协会。
Despite advances in the measurement of lymphatic function, little is known about the actual velocities of flow in microlymphatic (similar to 100 mu m diam) vessels. In this work, video microscopy and particle tracking methods are adapted and integrated with an ultra-highspeed imaging camera to obtain measurements of lymph velocities throughout the entire lymphatic contraction cycle in the rat mesentery, something that previous systems were incapable of measuring. To determine the system's accuracy, calibration experiments are conducted across the hypothesized physiologically significant range of velocities for microlymphatic flow (up to 15 mm/sec). The system shows high accuracy, less than 2% error, when comparing actual with measured velocities. Microspheres flowing through 140-mu m-diam tubing are imaged to demonstrate the system's ability to determine flow rates in these small vessels by measuring particle velocities. To demonstrate biological applicability, mesenteric microlymphatics in loops of the small intestine of three male Sprague-Dawley rats are exteriorized and imaged with the high-speed system at a rate of 500 frames/sec for several contraction sequences. Lymph velocity fluctuates cyclically with the vessel wall contractions, ranging from -1 to 7 mm/sec. These rates are higher than would be possible with standard video microscopy (3.75 mm/sec maximum). (C) 2005 Society of Photo-Optical Instrumentation Engineers.