Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages.
Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages.
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古细菌 DNA 分离机制的结构揭示了细菌和真核生物的联系。
DOI：
10.1126/science.aaa9046
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发表时间：
2015-09-04
期刊：
影响因子：
--
通讯作者：
Barillà D
中科院分区：
文献类型：
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作者：
Schumacher MA;Tonthat NK;Lee J;Rodriguez-Castañeda FA;Chinnam NB;Kalliomaa-Sanford AK;Ng IW;Barge MT;Shaw PL;Barillà D
尽管最近的研究提供了大量关于古细菌生物学的信息，但对这些生物中DNA分离的分子基础一无所知。本文揭示了古细菌Sulfolobus pNOB8分区系统的结构和组装机制。这个系统使用了三种蛋白质；ParA，一个非典型的ParB接头和着丝粒结合成分AspA。AspA利用一种扩散机制来创造一个DNA超螺旋，ParB在其上组装。这个超复合体连接到ParA马达，它包含一个类似细菌的Walker基元。ParB c结构域与CenpA具有结构相似性，这决定了真核生物的分离。因此，这个古细菌系统结合了类似细菌和类似真核生物的成分，表明在生命的三个领域中DNA分离原则可能是守恒的。
Although recent studies have provided a wealth of information about archaeal biology, nothing is known about the molecular basis of DNA segregation in these organisms. Here we unveil the machinery and assembly mechanism of the archaeal Sulfolobus pNOB8 partition system. This system employs three proteins; ParA, an atypical ParB adaptor and a centromere-binding component, AspA. AspA utilizes a spreading mechanism to create a DNA superhelix onto which ParB assembles. This supercomplex links to the ParA motor, which contains a bacteria-like Walker motif. The ParB C-domain harbors structural similarity to CenpA, which dictates eukaryotic segregation. Thus, this archaeal system combines bacteria-like and eukarya-like components, suggesting the possible conservation of DNA segregation principles across the three domains of life.