Loss of multipotency in adipose-derived stem cells after culture in temperature-responsive injectable polymer hydrogels
Loss of multipotency in adipose-derived stem cells after culture in temperature-responsive injectable polymer hydrogels
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DOI：
10.1038/s41428-022-00739-4
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发表时间：
2022-12
期刊：
影响因子：
2.8
通讯作者：
Nozomi Mayumi;Nobuo Murase;Yuta Yoshizaki;A. Kuzuya;Y. Ohya
中科院分区：
文献类型：
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作者：
Nozomi Mayumi;Nobuo Murase;Yuta Yoshizaki;A. Kuzuya;Y. Ohya
脂肪源性干细胞（AdSC）是一种间充质干细胞，有望应用于再生医学和细胞输送系统。细胞多能性的维持和分化方向的控制对于这些应用非常重要。然而，人们普遍认为这些细胞的分化方向取决于其支架的物理特性。在本研究中，我们探讨了当AdSC从具有不同交联程度的可注射聚合物（IP）水凝胶中取出并诱导分化为成骨细胞和脂肪细胞时，AdSC的多能性（即它们分化为多种细胞的能力）是否得以维持。我们证实在 IP 水凝胶中培养的 AdSC 保持未分化状态。然而，不能确保它们分化为成骨细胞和脂肪细胞；具体来说，当 AdSC 在 IP 水凝胶中培养时，它们的多能性可能会降低。当在极其柔软且细胞粘附特性差的 IP 水凝胶中培养时，AdSC 仍处于未分化状态，但其多能性降低。这些结果为使用 IP 水凝胶的干细胞递送系统提供了重要的见解。
Adipose-derived stem cells (AdSCs), a type of mesenchymal stem cell, are expected to be applicable to regenerative medicine and cellular delivery systems. The maintenance of cell multipotency and control of the differentiation direction are important for these applications. However, the differentiation direction of these cells is widely believed to depend on the physical properties of their scaffold. In this study, we explored whether the multipotency of AdSCs, that is, their ability to differentiate into multiple cells, is maintained when they are removed from injectable polymer (IP) hydrogels with various degrees of cross-linking and induced to differentiate into osteoblasts and adipocytes. We confirmed that AdSCs cultured in IP hydrogels maintained an undifferentiated state. However, their differentiation into osteoblasts and adipocytes cannot be ensured; specifically, the multipotency of AdSCs may decrease when they are cultured in IP hydrogels. When cultured in an IP hydrogel with extreme softness and poor cell adhesion properties, the AdSCs remained in an undifferentiated state, but their multipotency was reduced. These results provide important insights into stem cell delivery systems using IP hydrogels.