IMMUNOFLUORESCENT LOCALIZATION OF TYPE-IV COLLAGEN AND LAMININ DURING ENDOCHONDRAL BONE DIFFERENTIATION AND REGULATION BY PITUITARY GROWTH-HORMONE
IMMUNOFLUORESCENT LOCALIZATION OF TYPE-IV COLLAGEN AND LAMININ DURING ENDOCHONDRAL BONE DIFFERENTIATION AND REGULATION BY PITUITARY GROWTH-HORMONE
复制标题
DOI：
10.1016/0012-1606(80)90149-9
复制
发表时间：
1980-01-01
影响因子：
2.7
通讯作者：
REDDI, AH
中科院分区：
文献类型：
--
作者：
FOIDART, JM;REDDI, AH
研究了软骨内骨分化过程中的血管形成以及生长激素对该过程的影响。通过 2 种血管基底膜蛋白、IV 型胶原和层粘连蛋白（最近描述的一种糖蛋白）的免疫荧光定位来监测血管侵袭。通过因子 VIII 的定位来鉴定内皮细胞侵袭。皮下注射诱导新骨形成。植入脱矿大鼠骨基质粗粉。第1-9天，斑块中没有检测到血管成分。第3天出现间充质细胞，第7天增殖并分化为软骨，而斑块外围的毛细血管则增殖。从第 9 天开始，毛细血管侵入斑块中心，IV 型胶原、层粘连蛋白和因子 VIII 定位于侵入的血管内皮细胞中。 IV型胶原蛋白和层粘连蛋白同时出现在毛细血管基底膜中，随后出现在海绵窦的内皮层中。它们的分布模式是相同的。到第14天，血管侵犯就很明显。在垂体切除的大鼠中，软骨正常分化，但血管形成延迟和减少。 45Ca 掺入表明骨形成很少。对垂体切除的受者施用牛生长激素可将血管化和骨形成恢复到对照中观察到的水平。
Vascularization and the influence of growth hormone on this process were studied during endochondral bone differentiation. Vascular invasion was monitored by immunofluorescent localization of 2 vascular basement membrane proteins, type IV collagen and laminin, a recently described glycoprotein. Endothelial cell invasion was identified by localization of Factor VIII. New bone formation was induced by s.c. implantation of a coarse powder of demineralized rat bone matrix. On days 1-9, no vascular elements were detected in the plaque. Mesenchymal cells appeared on day 3, proliferated and differentiated into cartilage on day 7, while the capillaries proliferated at the periphery of the plaque. Beginning on day 9 with capillary incursion into the center of the plaque, type IV collagen, laminin and Factor VIII were localized in the invading vascular endothelial cells. Type IV collagen and laminin appeared synchronously in the capillary basement membranes and later in the endothelial lining of cavernous sinusoids. Their distribution pattern was identical. The vascular invasion was prominent by day 14. In hypophysectomized rats, cartilage differentiated normally but vascularization was delayed and reduced. Bone formation was scanty as indicated by 45Ca incorporation. Administration of bovine growth hormone to hypophysectomized recipients restored vascularization and bone formation to the level observed in controls.