Unscheduled DNA synthesis related to transformation grade of human urothelial cells.
Unscheduled DNA synthesis related to transformation grade of human urothelial cells.
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与人类尿路上皮细胞转化等级相关的非计划 DNA 合成。
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
J. Kieler
中科院分区：
文献类型：
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作者：
W. Bem;B. Christensen;J. Kieler
本文用定量放射自显影法研究了三个转化级别（TGr Ⅰ-Ⅲ）的人尿路上皮细胞的程序外DNA合成（UDS）。细胞孵育在补充有羟基脲的无精氨酸培养基中显示剂量依赖性UDS后，管理剂损伤DNA，而预定的DNA合成几乎完全抑制。用最终致癌物N-甲基-N-亚硝基-N '-硝基胍（CAS：70-25-7）或前致癌物4-硝基喹啉-1-氧化物（4-NQO; CAS：56-57-5）处理后，证实了UDS。在用苯并[a]芘（CAS：50-32-8）处理的培养物中，其需要与4-NQO不同的活化系统，UDS不太明显。在9个细胞系中，UDS的平均比率与TGr呈负相关。两种细胞系显示出不同的模式。
Unscheduled DNA synthesis (UDS) was studied by quantitative autoradiography in human urothelial cells of three transformation grades (TGr I-III). Cells incubated in arginine-free medium supplemented with hydroxyurea showed dose-dependent UDS after administration of agents injurious to DNA, while the scheduled synthesis of DNA was nearly totally suppressed. UDS was demonstrated after treatment with the ultimate carcinogen N-methyl-N-nitroso-N'-nitroguanidine (CAS: 70-25-7) or with the procarcinogen 4-nitroquinoline-1-oxide (4-NQO; CAS: 56-57-5). In cultures treated with benzo[a]pyrene (CAS: 50-32-8), which requires a different activation system than that for 4-NQO, UDS was less pronounced. In 9 cell lines the average rates of UDS were inversely related to TGr. Two cell lines showed a different pattern.