Subsurface tissue lesions created using an Nd:YAG laser and a sapphire contact cooling probe
Subsurface tissue lesions created using an Nd:YAG laser and a sapphire contact cooling probe
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DOI：
10.1002/lsm.20112
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发表时间：
2004-01-01
影响因子：
2.4
通讯作者：
Hayman, MH
中科院分区：
文献类型：
--
作者：
Ramli, R;Chung, CC;Hayman, MH
背景和目的：本研究探讨了组织深度激光凝固结合组织表面接触冷却在泌尿外科无创手术中的潜在应用。研究设计/材料与方法：设计并测试了一种激光探针，用于Nd:YAG激光同时照射和蓝宝石接触冷却肝脏和皮肤组织样品，离体。肉眼和组织学检查用于量化热病变的尺寸。结果：肝脏病变直径为5.5 +/- 0.3 mm，保留组织表面深度为2.1 +/- 0.2 mm （n = 5）。皮肤病变直径为4.3 +/- 0.9 mm，皮肤表面深度为1.1 +/- 0.2 mm （n = 6）。对于给定组织类型，接触冷却（蓝宝石）与非接触冷却（冷冻剂喷雾）在病变直径和保存组织层数上无统计学差异（P < 0.05）。结论：针对组织结构在组织表面几毫米内进行热凝固的无创激光手术是可行的，并可能扩大激光/冷却联合技术在泌尿外科和普通外科的应用。目前正在进行体内动物研究，以优化激光和冷却参数，以用于潜在的临床应用。
Background and Objectives: This study investigates deep laser coagulation of tissue in combination with contact cooling of the tissue surface for potential use in noninvasive procedures in urology.Study Design/Materials and Methods: A laser probe was designed and tested for simultaneous Nd:YAG laser irradiation and sapphire contact cooling of liver and skin tissue samples, ex vivo. Gross and histologic examination was used to quantify thermal lesion dimensions.Results: Liver lesions measured 5.5 +/- 0.3 mm in diameter, while preserving the tissue surface to a depth of 2.1 +/- 0.2 mm (n = 5). Skin lesions measured 4.3 +/- 0.9 mm in diameter, while preserving the skin surface to a depth of 1.1 +/- 0.2 mm (n = 6). There were no statistical differences in lesion diameter and layer of preserved tissue between contact (sapphire) cooling and prior non-contact (cryogen spray) cooling results for a given tissue type (P > 0.05).Conclusions: Noninvasive laser procedures targeting tissue structures for thermal coagulation within a few millimeters of the tissue surface are feasible and may expand the use of combined laser/cooling techniques for applications in urology and general surgery. In vivo animal studies are currently in development to optimize the laser and cooling parameters for potential clinical applications.